Lu Guo1,2, Luyao Zhang3, Yan Guan1, Yan Li1, Chufeng Zhang1, Qisen Guo1. 1. Shandong Cancer Hospital and Institute, Shandong University, Shandong First Medical University, Shandong Academy of Medical Sciences, Jinan, China. 2. Oncology, Affiliated Hospital of Weifang Medical University, Weifang, China. 3. Breast and Thyroid Surgery, Affiliated Hospital of Weifang Medical University, Weifang, China.
Abstract
BACKGROUND: To study in vitro the effects of anlotinib combined with radiotherapy on the lung cancer H520 cell cycle and apoptosis. METHODS: The log growth period H520 cells were divided into the control group, anlotinib group (A group), radiotherapy group (RT group) and combined group (A + RT group). Cell cycle and apoptosis were detected by flow cytometry and changes in H520 cell cycle and apoptosis were analyzed in each group. RESULTS: Anlotinib was determined to significantly inhibit cell growth in all groups, both alone, or in combination with radiotherapy. After receiving corresponding treatments, the proportions of G2/M-phase cells in the control group, A group, RT group and A + RT group were different, and statistically significant (F = 32.086, P < 0.001). The apoptotic cell statistics of H520 cells in the control group, A group, RT group and A + RT group were significantly different (F = 44.537, P < 0.01). The relative expression of CDK1 in each group of cells was 0.04 ± 0.02, 0.07 ± 0.12, 0.81 ± 0.11, and 0.56 ± 0.16, respectively. There were differences between the groups by analysis of variance which were statistically significant (F = 58.36, P < 0.0001). The relative expression of cycle B in each group of cells was 0.27 ± 0.05, 0.40 ± 0.16, 0.65 ± 0.14, and 0.57 ± 0.13, respectively. There were differences between the groups by analysis of variance which were statistically significant (F = 10.77, P = 0.0002). CONCLUSIONS: Anlotinib had an inhibitory effect on lung cancer H520 cell proliferation. A higher rate of apoptosis and G2/M phase block was observed in the anlotinib-radiotherapy combined group. Anlotinib combined with radiotherapy was able to synergistically inhibit tumor cell growth. KEY POINTS: Anrotinib combined with radiotherapy can synergistically inhibit tumor cell growth.
BACKGROUND: To study in vitro the effects of anlotinib combined with radiotherapy on the lung cancerH520 cell cycle and apoptosis. METHODS: The log growth period H520 cells were divided into the control group, anlotinib group (A group), radiotherapy group (RT group) and combined group (A + RT group). Cell cycle and apoptosis were detected by flow cytometry and changes in H520 cell cycle and apoptosis were analyzed in each group. RESULTS:Anlotinib was determined to significantly inhibit cell growth in all groups, both alone, or in combination with radiotherapy. After receiving corresponding treatments, the proportions of G2/M-phase cells in the control group, A group, RT group and A + RT group were different, and statistically significant (F = 32.086, P < 0.001). The apoptotic cell statistics of H520 cells in the control group, A group, RT group and A + RT group were significantly different (F = 44.537, P < 0.01). The relative expression of CDK1 in each group of cells was 0.04 ± 0.02, 0.07 ± 0.12, 0.81 ± 0.11, and 0.56 ± 0.16, respectively. There were differences between the groups by analysis of variance which were statistically significant (F = 58.36, P < 0.0001). The relative expression of cycle B in each group of cells was 0.27 ± 0.05, 0.40 ± 0.16, 0.65 ± 0.14, and 0.57 ± 0.13, respectively. There were differences between the groups by analysis of variance which were statistically significant (F = 10.77, P = 0.0002). CONCLUSIONS:Anlotinib had an inhibitory effect on lung cancerH520 cell proliferation. A higher rate of apoptosis and G2/M phase block was observed in the anlotinib-radiotherapy combined group. Anlotinib combined with radiotherapy was able to synergistically inhibit tumor cell growth. KEY POINTS: Anrotinib combined with radiotherapy can synergistically inhibit tumor cell growth.
Authors: Wojciech Michowski; Joel M Chick; Chen Chu; Aleksandra Kolodziejczyk; Yichen Wang; Jan M Suski; Brian Abraham; Lars Anders; Daniel Day; Lukas M Dunkl; Mitchell Li Cheong Man; Tian Zhang; Phatthamon Laphanuwat; Nickolas A Bacon; Lijun Liu; Anne Fassl; Samanta Sharma; Tobias Otto; Emanuelle Jecrois; Richard Han; Katharine E Sweeney; Samuele Marro; Marius Wernig; Yan Geng; Alan Moses; Cheng Li; Steven P Gygi; Richard A Young; Piotr Sicinski Journal: Mol Cell Date: 2020-04-01 Impact factor: 17.970