Noha A Hassuna1, Mohamed El Feky2, Aliae A R Mohamed Hussein3, Manal A Mahmoud3, Naglaa K Idriss4, Sayed F Abdelwahab1,5, Maggie A Ibrahim2. 1. Department of Medical Microbiology and Immunology, Faculty of Medicine, Minia University, Minia, Egypt. 2. Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt. 3. Department of Chest Diseases and Tuberculosis, Faculty of Medicine, Assiut University, Assiut, Egypt. 4. Department of Medical Biochemistry, Faculty of Medicine, Assiut University, Assiut, Egypt. 5. Division of Pharmaceutical Microbiology, Department of Pharmaceutics and Industrial Pharmacy, Taif College of Pharmacy, Taif University, Taif, Saudi Arabia.
Abstract
BACKGROUND: Interleukin-18 (IL-18) and interferon-γ (IFN-γ) are cytokines of crucial role in inflammation and immune reactions. There is a growing evidence supporting important roles for IL-18 and IFN γ in tuberculosis (TB) infection and anti-tuberculosis immunity. OBJECTIVE: To evaluate the role of polymorphisms in IL-18-607 and -137 and INF-γ +874 in susceptibility to TB infection among Egyptian patients. METHODS: A case control study was conducted to investigate the polymorphism at IL-18-607, -137 and INF-γ+874 by sequence specific primer-polymerase chain reaction (SSP- PCR) in 105 patients with pulmonary and extra pulmonary tuberculosis and 106 controls. RESULTS: A significant protective effect against TB was found in homozygous CC genotype at IL-18 -137G/C, in addition to a 7-fold risk with GG and GC genotypes in the recessive model. Apart from a decreased risk with the AC genotype, no association was detected between the susceptibility to TB and different genotypes or alleles at the IL-18 -607A/C site. The homozygous AA genotype in INF-γ+874 showed a significant higher risk to TB than the homozygous TT or heterozygous AT genotypes with nearly a 2-fold risk of TB infection with the A allele. Regarding haplotype association, the GC haplotype was strongly associated with TB infection compared to other haplotypes. CONCLUSION: These findings suggest; for the first time in Egypt; a significant risk to TB infection with SNP at the IL-18-137G/C with no LD with SNP at the IL-18-607 site. The homozygous AA genotype in INF-γ+874 showed a significant higher risk to TB than the homozygous TT or heterozygous AT genotypes.
BACKGROUND:Interleukin-18 (IL-18) and interferon-γ (IFN-γ) are cytokines of crucial role in inflammation and immune reactions. There is a growing evidence supporting important roles for IL-18 and IFN γ in tuberculosis (TB) infection and anti-tuberculosis immunity. OBJECTIVE: To evaluate the role of polymorphisms in IL-18-607 and -137 and INF-γ +874 in susceptibility to TB infection among Egyptian patients. METHODS: A case control study was conducted to investigate the polymorphism at IL-18-607, -137 and INF-γ+874 by sequence specific primer-polymerase chain reaction (SSP- PCR) in 105 patients with pulmonary and extra pulmonary tuberculosis and 106 controls. RESULTS: A significant protective effect against TB was found in homozygous CC genotype at IL-18 -137G/C, in addition to a 7-fold risk with GG and GC genotypes in the recessive model. Apart from a decreased risk with the AC genotype, no association was detected between the susceptibility to TB and different genotypes or alleles at the IL-18 -607A/C site. The homozygous AA genotype in INF-γ+874 showed a significant higher risk to TB than the homozygous TT or heterozygous AT genotypes with nearly a 2-fold risk of TB infection with the A allele. Regarding haplotype association, the GC haplotype was strongly associated with TB infection compared to other haplotypes. CONCLUSION: These findings suggest; for the first time in Egypt; a significant risk to TB infection with SNP at the IL-18-137G/C with no LD with SNP at the IL-18-607 site. The homozygous AA genotype in INF-γ+874 showed a significant higher risk to TB than the homozygous TT or heterozygous AT genotypes.
Authors: Ludovica Segat; Daiana Bevilacqua; Michele Boniotto; Luiz C Arraes; Paulo R de Souza; Josè L de Lima Filho; Sergio Crovella Journal: Immunogenetics Date: 2006-05-05 Impact factor: 2.846
Authors: Anne O'Garra; Paul S Redford; Finlay W McNab; Chloe I Bloom; Robert J Wilkinson; Matthew P R Berry Journal: Annu Rev Immunol Date: 2013 Impact factor: 28.527