| Literature DB >> 33397967 |
Qiling Zhou1,2, Miao Yu3, Roberto Tirado-Magallanes1, Bin Li4, Lingshi Kong1, Mingrui Guo1,2, Zi Hui Tan1, Sanghoon Lee1,5, Li Chai6, Akihiko Numata1,7, Touati Benoukraf1,8, Melissa Jane Fullwood1,9,10, Motomi Osato1, Bing Ren4,11, Daniel G Tenen12,13.
Abstract
CCCTC binding factor (CTCF) is an important factor in the maintenance of chromatin-chromatin interactions, yet the mechanism regulating its binding to chromatin is unknown. We demonstrate that zinc finger protein 143 (ZNF143) is a key regulator for CTCF-bound promoter-enhancer loops. In the murine genome, a large percentage of CTCF and ZNF143 DNA binding motifs are distributed 37 bp apart in the convergent orientation. Furthermore, deletion of ZNF143 leads to loss of CTCF binding on promoter and enhancer regions associated with gene expression changes. CTCF-bound promoter-enhancer loops are also disrupted after excision of ZNF143. ZNF143-CTCF-bound promoter-enhancer loops regulate gene expression patterns essential for maintenance of murine hematopoietic stem and progenitor cell integrity. Our data suggest a common feature of gene regulation is that ZNF143 is a critical factor for CTCF-bound promoter-enhancer loops.Entities:
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Year: 2021 PMID： 33397967 PMCID： PMC7782510 DOI： 10.1038/s41467-020-20282-1
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919