| Literature DB >> 33395580 |
Stefan Harmsen1, Emin Ilker Medine2, Maxim Moroz2, Fuad Nurili3, Jose Lobo2, Yiyu Dong4, Mezruh Turkekul5, Naga Vara Kishore Pillarsetty2, Richard Ting3, Vladimir Ponomarev6, Oguz Akin2, Omer Aras7.
Abstract
Adoptive cell transfer of targeted chimeric antigen receptor (CAR) T cells has emerged as a highly promising cancer therapy. The pharmacodynamic action or CAR T cells is closely related to their pharmacokinetic profile; because of this as well as the risk of non-specific action, it is important to monitor their biodistribution and fate following infusion. To this end, we developed a dual-modal PET/near infrared fluorescent (NIRF) nanoparticle-based imaging agent for non-genomic labeling of human CAR T cells. Since the PET/NIRF nanoparticles did not affect cell viability or cytotoxic functionality and enabled long-term whole-body CAR T cell tracking using PET and NIRF in an ovarian peritoneal carcinomatosis model, this platform is a viable imaging technology to be applied in other cancer models.Entities:
Keywords: Dual-modal; Immune cell labeling; Nanomedicine; Near-infrared fluorescence; PET; Tracking
Mesh：
Year: 2020 PMID： 33395580 PMCID： PMC7870530 DOI： 10.1016/j.biomaterials.2020.120630
Source DB: PubMed Journal: Biomaterials ISSN： 0142-9612 Impact factor: 12.479