Yuyi Chen1,2, Qiudong Yang1,2, Chunhua Lv1,3, Yue Chen1,4, Wenhua Zhao1, Wenlei Li1, Hongyu Chen1, Hua Wang1, Wen Sun1,4, Hua Yuan1,2. 1. Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China. 2. Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China. 3. Department of Stomatology, Taizhou People's Hospital of Jiangsu Province, Taizhou, China. 4. Department of Basic Science of Stomatology, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China.
Abstract
OBJECTIVES: NLRP3 inflammasome is a critical part of the innate immune system and plays an important role in a variety of inflammatory diseases. However, the effects of NLRP3 inflammasome on periodontitis have not been fully studied. MATERIALS AND METHODS: We used ligature-induced periodontitis models of NLRP3 knockout mice (NLRP3KO ) and their wildtype (WT) littermates to compare their alveolar bone phenotypes. We further used Lysm-Cre/RosanTnG mouse to trace the changes of Lysm-Cre+ osteoclast precursors in ligature-induced periodontitis with or without MCC950 treatment. At last, we explored MCC950 as a potential drug for the treatment of periodontitis in vivo and in vitro. RESULTS: Here, we showed that the number of osteoclast precursors, osteoclast differentiation and alveolar bone loss were reduced in NLRP3KO mice compared with WT littermates, by using ligature-induced periodontitis model. Next, MCC950, a specific inhibitor of the NLRP3 inflammasome, was used to inhibit osteoclast precursors differentiation into osteoclast. Further, we used Lysm-Cre/RosanTnG mice to demonstrate that MCC950 decreases the number of Lysm-Cre+ osteoclast precursors in ligature-induced periodontitis. At last, treatment with MCC950 significantly suppressed alveolar bone loss with reduced IL-1β activation and osteoclast differentiation in ligature-induced periodontitis. CONCLUSION: Our findings reveal that NLRP3 regulates alveolar bone loss in ligature-induced periodontitis by promoting osteoclastic differentiation.
OBJECTIVES: NLRP3 inflammasome is a critical part of the innate immune system and plays an important role in a variety of inflammatory diseases. However, the effects of NLRP3 inflammasome on periodontitis have not been fully studied. MATERIALS AND METHODS: We used ligature-induced periodontitis models of NLRP3 knockout mice (NLRP3KO ) and their wildtype (WT) littermates to compare their alveolar bone phenotypes. We further used Lysm-Cre/RosanTnG mouse to trace the changes of Lysm-Cre+ osteoclast precursors in ligature-induced periodontitis with or without MCC950 treatment. At last, we explored MCC950 as a potential drug for the treatment of periodontitis in vivo and in vitro. RESULTS: Here, we showed that the number of osteoclast precursors, osteoclast differentiation and alveolar bone loss were reduced in NLRP3KO mice compared with WT littermates, by using ligature-induced periodontitis model. Next, MCC950, a specific inhibitor of the NLRP3 inflammasome, was used to inhibit osteoclast precursors differentiation into osteoclast. Further, we used Lysm-Cre/RosanTnG mice to demonstrate that MCC950 decreases the number of Lysm-Cre+ osteoclast precursors in ligature-induced periodontitis. At last, treatment with MCC950 significantly suppressed alveolar bone loss with reduced IL-1β activation and osteoclast differentiation in ligature-induced periodontitis. CONCLUSION: Our findings reveal that NLRP3 regulates alveolar bone loss in ligature-induced periodontitis by promoting osteoclastic differentiation.
Authors: Y Kawahara; T Kaneko; Y Yoshinaga; Y Arita; K Nakamura; C Koga; A Yoshimura; R Sakagami Journal: J Dent Res Date: 2020-03-23 Impact factor: 6.116
Authors: Priscila Cunha Nascimento; Micaele Maria Lopes Castro; Marcela Baraúna Magno; Anna Paula Costa Ponte Sousa Carvalho Almeida; Nathália Carolina Fernandes Fagundes; Lucianne Cople Maia; Rafael Rodrigues Lima Journal: Front Neurol Date: 2019-04-24 Impact factor: 4.003
Authors: C Carlomagno; D Bertazioli; A Gualerzi; S Picciolini; P I Banfi; A Lax; E Messina; J Navarro; L Bianchi; A Caronni; F Marenco; S Monteleone; C Arienti; M Bedoni Journal: Sci Rep Date: 2021-03-02 Impact factor: 4.379