Literature DB >> 33367518

Outbreak of Pseudomonas aeruginosa Infections from a Contaminated Gastroscope Detected by Whole Genome Sequencing Surveillance.

Alexander J Sundermann1,2, Jieshi Chen3, James K Miller3, Melissa I Saul4, Kathleen A Shutt1,2, Marissa P Griffith1,2, Mustapha M Mustapha1,2, Chinelo Ezeonwuka1,2, Kady Waggle1,2, Vatsala Srinivasa1,2, Praveen Kumar5, A William Pasculle6, Ashley M Ayres7, Graham M Snyder2,7, Vaughn S Cooper8, Daria Van Tyne2, Jane W Marsh1,2, Artur W Dubrawski3, Lee H Harrison1,2.   

Abstract

BACKGROUND: Traditional methods of outbreak investigations utilize reactive whole genome sequencing (WGS) to confirm or refute the outbreak. We have implemented WGS surveillance and a machine learning (ML) algorithm for the electronic health record (EHR) to retrospectively detect previously unidentified outbreaks and to determine the responsible transmission routes.
METHODS: We performed WGS surveillance to identify and characterize clusters of genetically-related Pseudomonas aeruginosa infections during a 24-month period. ML of the EHR was used to identify potential transmission routes. A manual review of the EHR was performed by an infection preventionist to determine the most likely route and results were compared to the ML algorithm.
RESULTS: We identified a cluster of 6 genetically related P. aeruginosa cases that occurred during a 7-month period. The ML algorithm identified gastroscopy as a potential transmission route for 4 of the 6 patients. Manual EHR review confirmed gastroscopy as the most likely route for 5 patients. This transmission route was confirmed by identification of a genetically-related P. aeruginosa incidentally cultured from a gastroscope used on 4of the 5 patients. Three infections, 2 of which were blood stream infections, could have been prevented if the ML algorithm had been running in real-time.
CONCLUSIONS: WGS surveillance combined with a ML algorithm of the EHR identified a previously undetected outbreak of gastroscope-associated P. aeruginosa infections. These results underscore the value of WGS surveillance and ML of the EHR for enhancing outbreak detection in hospitals and preventing serious infections.
© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

Entities:  

Keywords:  zzm321990 Pseudomonas aeruginosazzm321990 ; healthcare-associated infections; machine learning; outbreak detection; whole genome sequencing surveillance

Mesh:

Year:  2021        PMID: 33367518      PMCID: PMC8326550          DOI: 10.1093/cid/ciaa1887

Source DB:  PubMed          Journal:  Clin Infect Dis        ISSN: 1058-4838            Impact factor:   9.079


  20 in total

1.  SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing.

Authors:  Anton Bankevich; Sergey Nurk; Dmitry Antipov; Alexey A Gurevich; Mikhail Dvorkin; Alexander S Kulikov; Valery M Lesin; Sergey I Nikolenko; Son Pham; Andrey D Prjibelski; Alexey V Pyshkin; Alexander V Sirotkin; Nikolay Vyahhi; Glenn Tesler; Max A Alekseyev; Pavel A Pevzner
Journal:  J Comput Biol       Date:  2012-04-16       Impact factor: 1.479

Review 2.  Whole Genome Sequencing-Implications for Infection Prevention and Outbreak Investigations.

Authors:  Kyle J Popovich; Evan S Snitkin
Journal:  Curr Infect Dis Rep       Date:  2017-04       Impact factor: 3.725

3.  Gastroscopy-associated transmission of extended-spectrum beta-lactamase-producing Pseudomonas aeruginosa.

Authors:  O Bajolet; D Ciocan; C Vallet; C de Champs; V Vernet-Garnier; T Guillard; L Brasme; G Thiefin; G Cadiot; F Bureau-Chalot
Journal:  J Hosp Infect       Date:  2013-01-20       Impact factor: 3.926

4.  Prokka: rapid prokaryotic genome annotation.

Authors:  Torsten Seemann
Journal:  Bioinformatics       Date:  2014-03-18       Impact factor: 6.937

5.  Outbreak of Vancomycin-resistant Enterococcus faecium in Interventional Radiology: Detection Through Whole-genome Sequencing-based Surveillance.

Authors:  Alexander J Sundermann; Ahmed Babiker; Jane W Marsh; Kathleen A Shutt; Mustapha M Mustapha; Anthony W Pasculle; Chinelo Ezeonwuka; Melissa I Saul; Marissa P Pacey; Daria Van Tyne; Ashley M Ayres; Vaughn S Cooper; Graham M Snyder; Lee H Harrison
Journal:  Clin Infect Dis       Date:  2020-05-23       Impact factor: 9.079

6.  Bronchoscope-associated clusters of multidrug-resistant Pseudomonas aeruginosa and carbapenem-resistant Klebsiella pneumoniae.

Authors:  Alison L Galdys; Jane W Marsh; Edgar Delgado; A William Pasculle; Marissa Pacey; Ashley M Ayres; Amy Metzger; Lee H Harrison; Carlene A Muto
Journal:  Infect Control Hosp Epidemiol       Date:  2018-11-19       Impact factor: 3.254

7.  Transmission of Clostridioides difficile infection (CDI) from patients less than 3 years of age in a pediatric oncology setting.

Authors:  Elizabeth Robilotti; Weihua Huang; N Esther Babady; Donald Chen; Mini Kamboj
Journal:  Infect Control Hosp Epidemiol       Date:  2020-02       Impact factor: 3.254

8.  Multistate point-prevalence survey of health care-associated infections.

Authors:  Shelley S Magill; Jonathan R Edwards; Wendy Bamberg; Zintars G Beldavs; Ghinwa Dumyati; Marion A Kainer; Ruth Lynfield; Meghan Maloney; Laura McAllister-Hollod; Joelle Nadle; Susan M Ray; Deborah L Thompson; Lucy E Wilson; Scott K Fridkin
Journal:  N Engl J Med       Date:  2014-03-27       Impact factor: 91.245

9.  Complex Routes of Nosocomial Vancomycin-Resistant Enterococcus faecium Transmission Revealed by Genome Sequencing.

Authors:  Kathy E Raven; Theodore Gouliouris; Hayley Brodrick; Francesc Coll; Nicholas M Brown; Rosy Reynolds; Sandra Reuter; M Estée Török; Julian Parkhill; Sharon J Peacock
Journal:  Clin Infect Dis       Date:  2017-04-01       Impact factor: 9.079

10.  Draft Genome Sequences of Four Hospital-Associated Pseudomonas putida Isolates.

Authors:  Mustapha M Mustapha; Jane W Marsh; Chinelo D Ezeonwuka; Anthony W Pasculle; Marissa P Pacey; Ashley M Querry; Carlene A Muto; Lee H Harrison
Journal:  Genome Announc       Date:  2016-09-29
View more
  5 in total

1.  Genomic Diversity of Hospital-Acquired Infections Revealed through Prospective Whole-Genome Sequencing-Based Surveillance.

Authors:  Mustapha M Mustapha; Vatsala R Srinivasa; Marissa P Griffith; Shu-Ting Cho; Daniel R Evans; Kady Waggle; Chinelo Ezeonwuka; Daniel J Snyder; Jane W Marsh; Lee H Harrison; Vaughn S Cooper; Daria Van Tyne
Journal:  mSystems       Date:  2022-06-13       Impact factor: 7.324

2.  Whole-Genome Sequencing Surveillance and Machine Learning of the Electronic Health Record for Enhanced Healthcare Outbreak Detection.

Authors:  Alexander J Sundermann; Jieshi Chen; Praveen Kumar; Ashley M Ayres; Shu Ting Cho; Chinelo Ezeonwuka; Marissa P Griffith; James K Miller; Mustapha M Mustapha; A William Pasculle; Melissa I Saul; Kathleen A Shutt; Vatsala Srinivasa; Kady Waggle; Daniel J Snyder; Vaughn S Cooper; Daria Van Tyne; Graham M Snyder; Jane W Marsh; Artur Dubrawski; Mark S Roberts; Lee H Harrison
Journal:  Clin Infect Dis       Date:  2022-08-31       Impact factor: 20.999

Review 3.  Gastrointestinal endoscope contamination rates - elevators are not only to blame: a systematic review and meta-analysis.

Authors:  Hemant Goyal; Sara Larsen; Abhilash Perisetti; Nikolaj Birk Larsen; Lotte Klinten Ockert; Sven Adamsen; Benjamin Tharian; Nirav Thosani
Journal:  Endosc Int Open       Date:  2022-06-10

4.  mSphere of Influence: Whole-Genome Sequencing, a Vital Tool for the Interruption of Nosocomial Transmission.

Authors:  Ahmed Babiker
Journal:  mSphere       Date:  2021-04-28       Impact factor: 4.389

5.  New Variants of Pseudomonas aeruginosa High-Risk Clone ST233 Associated with an Outbreak in a Mexican Paediatric Hospital.

Authors:  Pamela Aguilar-Rodea; Elia L Estrada-Javier; Verónica Jiménez-Rojas; Uriel Gomez-Ramirez; Carolina G Nolasco-Romero; Gerardo E Rodea; Benjamín Antonio Rodríguez-Espino; Sandra Mendoza-Elizalde; Cesar Arellano; Beatriz López-Marcelino; Daniela de la Rosa Zamboni; Ana Estela Gamiño-Arroyo; Rosalia Mora-Suárez; Margarita Torres García; Isabel Franco Hernández; Israel Parra-Ortega; Guillermina Campos-Valdez; Norma Velázquez-Guadarrama; Irma Rosas-Pérez
Journal:  Microorganisms       Date:  2022-07-29
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.