| Literature DB >> 33257860 |
Taiko Kim To1, Yuichiro Nishizawa2, Soichi Inagaki2,3,4, Yoshiaki Tarutani3, Sayaka Tominaga2, Atsushi Toyoda5, Asao Fujiyama5, Frédéric Berger6, Tetsuji Kakutani7,8.
Abstract
DNA methylation is important for silencing transposable elements (TEs) in diverse eukaryotes, including plants. In plant genomes, TEs are silenced by methylation of histone H3 lysine 9 (H3K9) and cytosines in both CG and non-CG contexts. The role of RNA interference (RNAi) in establishing TE-specific silent marks has been extensively studied, but the importance of RNAi-independent pathways remains largely unexplored. Here, we directly investigated transgenerational de novo DNA methylation of TEs after the loss of silent marks. Our analyses uncovered potent and precise RNAi-independent pathways for recovering non-CG methylation and H3K9 methylation in most TE genes (that is, coding regions within TEs). Characterization of a subset of TE genes without the recovery revealed the effects of H3K9 demethylation, replacement of histone H2A variants and their interaction with CG methylation, together with feedback from transcription. These chromatin components are conserved among eukaryotes and may contribute to chromatin reprogramming in a conserved manner.Entities:
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Year: 2020 PMID： 33257860 DOI： 10.1038/s41477-020-00810-z
Source DB: PubMed Journal: Nat Plants ISSN： 2055-0278 Impact factor: 15.793