| Literature DB >> 33159251 |
Yoonjoo K Lee1, Kristen L Segars1, Vickery Trinkaus-Randall2.
Abstract
Chemical indicators are used to study calcium signaling events in the context of live cell imaging. Fluo-3 AM, Fluo-4 AM, and Cal-520 AM are three commonly used fluorescent indicators derived from the calcium chelator BAPTA. Here we describe sample protocols that detail how these indicators are used in in vitro and ex vivo experiments to analyze the role of calcium mobilizations in cell-cell communication and coordinated cellular motility in the context of wound healing.Entities:
Keywords: Calcium mobilization; Cell-cell communication; Confocal imaging; Ex vivo in situ analysis; Image analysis; Live cell imaging
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Year: 2021 PMID： 33159251 PMCID： PMC8787922 DOI： 10.1007/7651_2020_329
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745