Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Mammalian kinetochore/centromere composition: a 50 kDa antigen is present in the mammalian kinetochore/centromere.

Literature DB >> 3315496

Mammalian kinetochore/centromere composition: a 50 kDa antigen is present in the mammalian kinetochore/centromere.

Abstract

The composition of the mammalian kinetochore/centromere was studied by indirect immunofluorescence and immunoblotting protocols using serum from a patient with the CREST variant of scleroderma. The results of these studies suggest that a protein with a molecular weight of 50 kDa is localized at the surface of the primary constrictions (the kinetochore region) of both human and Indian muntjac chromosomes. In addition, we were able to verify the presence of a 19.5 kDa antigen (CENP-A), previously detected in human centromeres, within the kinetochore region of the Indian muntjac. These data suggest that the composition of the kinetochore region of the primary constriction is complex and that there is conservation in composition within the mammals. These features may reflect the important role of this unique chromosomal domain in the maintenance of ploidy.

Entities: Disease Gene Species

Mesh：

Substances：
Antigens
Nuclear Proteins

Year: 1987 PMID： 3315496 DOI： 10.1007/BF00333991

Source DB: PubMed Journal: Chromosoma ISSN： 0009-5915 Impact factor: 4.316

28 in total

1. Sites of microtubule assembly and disassembly in the mitotic spindle.

Authors: T Mitchison; L Evans; E Schulze; M Kirschner
Journal: Cell Date: 1986-05-23 Impact factor: 41.582

2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

3. The ultrastructure and spatial organization of the metaphase kinetochore in mitotic rat cells.

Authors: P T Jokelainen
Journal: J Ultrastruct Res Date: 1967-07

4. Three human chromosomal autoantigens are recognized by sera from patients with anti-centromere antibodies.

Authors: W Earnshaw; B Bordwell; C Marino; N Rothfield
Journal: J Clin Invest Date: 1986-02 Impact factor: 14.808

5. Fine structure of kinetochore in Indian muntjac.

Authors: D E Comings; T A Okada
Journal: Exp Cell Res Date: 1971-07 Impact factor: 3.905

6. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots.

Authors: M S Blake; K H Johnston; G J Russell-Jones; E C Gotschlich
Journal: Anal Biochem Date: 1984-01 Impact factor: 3.365

7. Anticentromere antibodies bind to trout testis histone 1 and a low molecular weight protein from rabbit thymus.

Authors: L M Ayer; M J Fritzler
Journal: Mol Immunol Date: 1984-09 Impact factor: 4.407

8. Human anti-centromere sera recognise a 19.5 kD non-histone chromosomal protein from HeLa cells.

Authors: H H Guldner; H J Lakomek; F A Bautz
Journal: Clin Exp Immunol Date: 1984-10 Impact factor: 4.330

9. Human anticentromere antibodies: distribution, characterization of antigens, and effect on microtubule organization.

Authors: J V Cox; E A Schenk; J B Olmsted
Journal: Cell Date: 1983-11 Impact factor: 41.582

10. The CREST syndrome: a distinct serologic entity with anticentromere antibodies.

Authors: M J Fritzler; T D Kinsella
Journal: Am J Med Date: 1980-10 Impact factor: 4.965

33 in total

1. A 47-kDa human nuclear protein recognized by antikinetochore autoimmune sera is homologous with the protein encoded by RCC1, a gene implicated in onset of chromosome condensation.

Authors: F R Bischoff; G Maier; G Tilz; H Ponstingl
Journal: Proc Natl Acad Sci U S A Date: 1990-11 Impact factor: 11.205

2. MSA-36: a chromosomal and mitotic spindle-associated protein.

Authors: J B Rattner; T Wang; G Mack; M J Fritzler; L Martin; D Valencia
Journal: Chromosoma Date: 1992-10 Impact factor: 4.316

3. Purification of the centromere-specific protein CENP-A and demonstration that it is a distinctive histone.

Authors: D K Palmer; K O'Day; H L Trong; H Charbonneau; R L Margolis
Journal: Proc Natl Acad Sci U S A Date: 1991-05-01 Impact factor: 11.205

Review 4. Kinetochore fiber formation in animal somatic cells: dueling mechanisms come to a draw.

Authors: Conly L Rieder
Journal: Chromosoma Date: 2005-11-12 Impact factor: 4.316

Review 5. The ABCs of CENPs.

Authors: Marinela Perpelescu; Tatsuo Fukagawa
Journal: Chromosoma Date: 2011-07-13 Impact factor: 4.316

Review 6. Autoantibodies to components of the mitotic apparatus.

Authors: J B Rattner; G J Mack; M J Fritzler
Journal: Mol Biol Rep Date: 1998-07 Impact factor: 2.316

7. An antigen located in the kinetochore region in metaphase and on polar microtubule ends in the midbody region in anaphase, characterised using a monoclonal antibody.

Authors: R Pankov; M Lemieux; R Hancock
Journal: Chromosoma Date: 1990-04 Impact factor: 4.316