Literature DB >> 2023923

Purification of the centromere-specific protein CENP-A and demonstration that it is a distinctive histone.

D K Palmer1, K O'Day, H L Trong, H Charbonneau, R L Margolis.   

Abstract

CENP-A, a centromere-specific 17-kDa protein, has histone-like properties. However, in contrast to the common somatic histones, CENP-A is quantitatively retained in bull spermatozoa, and we have exploited this fact to purify CENP-A to apparent homogeneity. Partial sequence analysis of the purified protein indicates that CENP-A is a distinctive gene product. Some CENP-A sequences are highly similar to regions of histone H3. Other segments of CENP-A are not related to H3 or any other histone. These unrelated segments are presumably involved in localizing CENP-A to centromeric DNA or in centromere-specific functions of CENP-A.

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Year:  1991        PMID： 2023923      PMCID： PMC51527          DOI： 10.1073/pnas.88.9.3734

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  29 in total

1.  The centromere specific histone CENP-A is selectively retained in discrete foci in mammalian sperm nuclei.

Authors:  D K Palmer; K O'Day; R L Margolis
Journal:  Chromosoma       Date:  1990-12       Impact factor: 4.316

2.  Identification of a family of human centromere proteins using autoimmune sera from patients with scleroderma.

Authors:  W C Earnshaw; N Rothfield
Journal:  Chromosoma       Date:  1985       Impact factor: 4.316

3.  Histone 3. 3. Sequence studies on the cyanogen bromide peptides; complete amino acid sequence of calf thymus histone 3.

Authors:  R J DeLange; J A Hooper; E L Smith
Journal:  J Biol Chem       Date:  1973-05-10       Impact factor: 5.157

4.  The chromatographic determination of cystine and cysteine residues in proteins as s-beta-(4-pyridylethyl)cysteine.

Authors:  M Friedman; L H Krull; J F Cavins
Journal:  J Biol Chem       Date:  1970-08-10       Impact factor: 5.157

5.  Non-allelic variants of histones 2a, 2b and 3 in mammals.

Authors:  S G Franklin; A Zweidler
Journal:  Nature       Date:  1977-03-17       Impact factor: 49.962

6.  Controlled proteolysis of tubulin by subtilisin: localization of the site for MAP2 interaction.

Authors:  L Serrano; J Avila; R B Maccioni
Journal:  Biochemistry       Date:  1984-09-25       Impact factor: 3.162

7.  The cyclic tyrosination/detyrosination of alpha tubulin.

Authors:  W C Thompson
Journal:  Methods Cell Biol       Date:  1982       Impact factor: 1.441

8.  Human anti-centromere sera recognise a 19.5 kD non-histone chromosomal protein from HeLa cells.

Authors:  H H Guldner; H J Lakomek; F A Bautz
Journal:  Clin Exp Immunol       Date:  1984-10       Impact factor: 4.330

9.  Histone fractionation by high-performance liquid chromatography.

Authors:  L R Gurley; J G Valdez; D A Prentice; W D Spall
Journal:  Anal Biochem       Date:  1983-02-15       Impact factor: 3.365

10.  The kinetochore is part of the metaphase chromosome scaffold.

Authors:  W C Earnshaw; N Halligan; C Cooke; N Rothfield
Journal:  J Cell Biol       Date:  1984-01       Impact factor: 10.539
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  152 in total

1.  Early disruption of centromeric chromatin organization in centromere protein A (Cenpa) null mice.

Authors:  E V Howman; K J Fowler; A J Newson; S Redward; A C MacDonald; P Kalitsis; K H Choo
Journal:  Proc Natl Acad Sci U S A       Date:  2000-02-01       Impact factor: 11.205

2.  Spatial and temporal regulation of Condensins I and II in mitotic chromosome assembly in human cells.

Authors:  Takao Ono; Yuda Fang; David L Spector; Tatsuya Hirano
Journal:  Mol Biol Cell       Date:  2004-05-14       Impact factor: 4.138

3.  Localisation of centromeric proteins to a fraction of mouse minor satellite DNA on a mini-chromosome in human, mouse and chicken cells.

Authors:  Kang Zeng; Jose I de las Heras; Andrew Ross; Jian Yang; Howard Cooke; Ming Hong Shen
Journal:  Chromosoma       Date:  2004-07-28       Impact factor: 4.316

4.  Replication forks pause at yeast centromeres.

Authors:  S A Greenfeder; C S Newlon
Journal:  Mol Cell Biol       Date:  1992-09       Impact factor: 4.272

5.  Centromere activity in dicentric small supernumerary marker chromosomes.

Authors:  Elisabeth Ewers; Kinya Yoda; Ahmed B Hamid; Anja Weise; Marina Manvelyan; Thomas Liehr
Journal:  Chromosome Res       Date:  2010-06-22       Impact factor: 5.239

6.  Heterochromatic deposition of centromeric histone H3-like proteins.

Authors:  S Henikoff; K Ahmad; J S Platero; B van Steensel
Journal:  Proc Natl Acad Sci U S A       Date:  2000-01-18       Impact factor: 11.205

7.  The CNA1 histone of the ciliate Tetrahymena thermophila is essential for chromosome segregation in the germline micronucleus.

Authors:  Marcella D Cervantes; Xiaohui Xi; Danielle Vermaak; Meng-Chao Yao; Harmit S Malik
Journal:  Mol Biol Cell       Date:  2005-10-26       Impact factor: 4.138

8.  Ccp1 Homodimer Mediates Chromatin Integrity by Antagonizing CENP-A Loading.

Authors:  Qianhua Dong; Feng-Xiang Yin; Feng Gao; Yuan Shen; Faben Zhang; Yang Li; Haijin He; Marlyn Gonzalez; Jinpu Yang; Shu Zhang; Min Su; Yu-Hang Chen; Fei Li
Journal:  Mol Cell       Date:  2016-09-22       Impact factor: 17.970

9.  Cellular expression of human centromere protein C demonstrates a cyclic behavior with highest abundance in the G1 phase.

Authors:  M Knehr; M Poppe; D Schroeter; W Eickelbaum; E M Finze; U L Kiesewetter; M Enulescu; M Arand; N Paweletz
Journal:  Proc Natl Acad Sci U S A       Date:  1996-09-17       Impact factor: 11.205

10.  Functional complementation of human centromere protein A (CENP-A) by Cse4p from Saccharomyces cerevisiae.

Authors:  Gerhard Wieland; Sandra Orthaus; Sabine Ohndorf; Stephan Diekmann; Peter Hemmerich
Journal:  Mol Cell Biol       Date:  2004-08       Impact factor: 4.272
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