Literature DB >> 33153

Purification and properties of an inducible beta-galactosidase isolated from the yeast Kluyveromyces lactis.

R C Dickson, L R Dickson, J S Markin.   

Abstract

beta-Galactosidase (EC 3.2.1.32) was purified 80-fold from the yeast Kluyveromyces lactis induced for this enzyme by growth on lactose. When the purified enzyme was subjected to electrophoresis on an acrylamide gel in the presence of sodium dodecyl sulfate, one protein with an apparent molecular weight of 135,000 was observed. The enzyme has a sedimentation coefficient of 9.6S. This beta-galactosidase and the one from Escherichia coli are not antigenically related. Maximal enzyme activity requires Na+ and Mn2+ and a reducing agent. beta-Galactosidase has Km values of 12 to 17 and 1.6 mM for lactose and o-nitrophenyl-beta-D-galactoside, respectively. The hydrolase and transgalactosylase activities of the enzyme are similar to those of E. coli beta-galactosidase.

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Year:  1979        PMID： 33153      PMCID： PMC218417          DOI： 10.1128/jb.137.1.51-61.1979

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  21 in total

Review 1.  Methods for avoiding proteolytic artefacts in studies of enzymes and other proteins from yeasts.

Authors:  J R Pringle
Journal:  Methods Cell Biol       Date:  1975       Impact factor: 1.441

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  A quantitation of the factors which affect the hydrolase and transgalactosylase activities of beta-galactosidase (E. coli) on lactose.

Authors:  R E Huber; G Kurz; K Wallenfels
Journal:  Biochemistry       Date:  1976-05-04       Impact factor: 3.162

4.  The amino acid sequence of beta-galactosidase of Escherichia coli.

Authors:  A V Fowler; I Zabin
Journal:  Proc Natl Acad Sci U S A       Date:  1977-04       Impact factor: 11.205

5.  lac Repressor-operator interaction. VI. The natural inducer of the lac operon.

Authors:  A Jobe; S Bourgeois
Journal:  J Mol Biol       Date:  1972-08-28       Impact factor: 5.469

Review 6.  The statistical analysis of enzyme kinetic data.

Authors:  W W Cleland
Journal:  Adv Enzymol Relat Areas Mol Biol       Date:  1967

7.  Mutants in Saccharomyces lactis controlling both -glucosidase and -galactosidase activities.

Authors:  M Tingle; H O Halvorson
Journal:  Genet Res       Date:  1972-02       Impact factor: 1.588

8.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

9.  The purification of beta-galactosidase from Escherichia coli by affinity chromatography.

Authors:  E Steers; P Cuatrecasas; H B Pollard
Journal:  J Biol Chem       Date:  1971-01-10       Impact factor: 5.157

10.  Molecular cloning and expression in E. coli of a yeast gene coding for beta-galactosidase.

Authors:  R C Dickson; J S Markin
Journal:  Cell       Date:  1978-09       Impact factor: 41.582
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  15 in total

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Authors:  Wang Cheng; Lei Wang; Yong-Liang Jiang; Xiao-Hui Bai; Jun Chu; Qiong Li; Ge Yu; Qiu-Ling Liang; Cong-Zhao Zhou; Yuxing Chen
Journal:  J Biol Chem       Date:  2012-05-16       Impact factor: 5.157

2.  Transcriptional regulation of the Kluyveromyces lactis beta-galactosidase gene.

Authors:  L R Lacy; R C Dickson
Journal:  Mol Cell Biol       Date:  1981-07       Impact factor: 4.272

3.  Purification and characterisation of an inducible beta-galactosidase from Corynebacterium murisepticum.

Authors:  M Priyolkar; C K Nair; D S Pradhan
Journal:  Arch Microbiol       Date:  1989       Impact factor: 2.552

4.  Lac4 is the structural gene for beta-galactosidase in Kluyveromyces lactis.

Authors:  R M Sheetz; R C Dickson
Journal:  Genetics       Date:  1981-08       Impact factor: 4.562

5.  Genetic regulation: yeast mutants constitutive for beta-galactosidase activity have an increased level of beta-galactosidase messenger ribonucleic acid.

Authors:  R C Dickson; R M Sheetz; L R Lacy
Journal:  Mol Cell Biol       Date:  1981-11       Impact factor: 4.272

6.  Transport and hydrolysis of disaccharides by Trichosporon cutaneum.

Authors:  M Mörtberg; H Y Neujahr
Journal:  J Bacteriol       Date:  1986-11       Impact factor: 3.490

7.  Characterization of lactose transport in Kluyveromyces lactis.

Authors:  R C Dickson; K Barr
Journal:  J Bacteriol       Date:  1983-06       Impact factor: 3.490

8.  Location of the beta-galactosidase of the yeast Kluyveromyces marxianus var. marxianus ATCC 10022.

Authors:  M Bacci Júnior; C G Siqueira; S A Antoniazi; J Ueta
Journal:  Antonie Van Leeuwenhoek       Date:  1996-05       Impact factor: 2.271

9.  Physiological studies of beta-galactosidase induction in Kluyveromyces lactis.

Authors:  R C Dickson; J S Markin
Journal:  J Bacteriol       Date:  1980-06       Impact factor: 3.490

10.  Properties of beta-galactosidase III: implications for entry of galactosides into Klebsiella.

Authors:  B G Hall
Journal:  J Bacteriol       Date:  1980-05       Impact factor: 3.490
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