Literature DB >> 2492796

Purification and characterisation of an inducible beta-galactosidase from Corynebacterium murisepticum.

M Priyolkar1, C K Nair, D S Pradhan.   

Abstract

The beta-galactosidase (EC 3.2.1.32) of Corynebacterium murisepticum (inducible by lactose and galactose) was purified by successive column chromatography on Sephadex G-200, DEAE-Sephadex A-50 and DEAE-cellulose (DE52). The enzyme was found to be a dimer of identical subunits of molecular mass 100,000 daltons. The Km values of the enzyme for the substrates lactose and o-nitrophenyl-beta-D-galactopyranoside (ONPG) are 16.7 mM and 4.4 mM, respectively, indicating, its low affinity for the substrates. The Ouchterlony immunodiffusion method exhibited immunological homogeneity of the enzyme preparation. The catalytic site of the enzyme does not take part in antigen-antibody reaction.

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Year:  1989        PMID： 2492796     DOI： 10.1007/bf00444668

Source DB:  PubMed          Journal:  Arch Microbiol        ISSN： 0302-8933            Impact factor:   2.552


  18 in total

1.  PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12.

Authors:  G R CRAVEN; E STEERS; C B ANFINSEN
Journal:  J Biol Chem       Date:  1965-06       Impact factor: 5.157

2.  Characterization of Extracellular beta-d-Galactosidase from Fusarium moniliforme Grown in Whey.

Authors:  B J Macris; P Markakis
Journal:  Appl Environ Microbiol       Date:  1981-04       Impact factor: 4.792

3.  Beta-galactosidase activity and lactose utilization in Aspergillus nidulans.

Authors:  P A Fantes; C F Roberts
Journal:  J Gen Microbiol       Date:  1973-08

4.  Measurement of molecular weights by electrophoresis on SDS-acrylamide gel.

Authors:  K Weber; J R Pringle; M Osborn
Journal:  Methods Enzymol       Date:  1972       Impact factor: 1.600

5.  Molecular basis of isozyme formation of beta-galactosidases in Bacillus stearothermophilus: isolation of two beta-galactosidase genes, bgaA and bgaB.

Authors:  H Hirata; S Negoro; H Okada
Journal:  J Bacteriol       Date:  1984-10       Impact factor: 3.490

6.  Induction and general properties of beta-galactosidase and beta-galactoside permease in Pseudomonas BAL-31.

Authors:  C Hidalgo; J Reyes; R Goldschmidt
Journal:  J Bacteriol       Date:  1977-02       Impact factor: 3.490

7.  Plasmids in Streptococcus lactis: evidence that lactose metabolism and proteinase activity are plasmid linked.

Authors:  J D Efstathiou; L L McKay
Journal:  Appl Environ Microbiol       Date:  1976-07       Impact factor: 4.792

8.  BETA-GALACTOSIDASE OF STREPTOCOCCUS LACTIS.

Authors:  J E CITTI; W E SANDINE; P R ELLIKER
Journal:  J Bacteriol       Date:  1965-04       Impact factor: 3.490

9.  Induction and characterization of -galactosidase in an extreme thermophile.

Authors:  J T Ulrich; G A McFeters; K L Temple
Journal:  J Bacteriol       Date:  1972-05       Impact factor: 3.490

10.  A positive regulatory element is involved in the induction of the beta-galactosidase gene from Kluyveromyces lactis.

Authors:  S Das; K D Breunig; C P Hollenberg
Journal:  EMBO J       Date:  1985-03       Impact factor: 11.598
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  2 in total

1.  Simple Method To Detect beta-Galactosidase.

Authors:  T Bhowmik; E H Marth
Journal:  Appl Environ Microbiol       Date:  1989-12       Impact factor: 4.792

2.  Purification and characterization of a thermotolerant beta-galactosidase from Thermomyces lanuginosus.

Authors:  L Fischer; C Scheckermann; F Wagner
Journal:  Appl Environ Microbiol       Date:  1995-04       Impact factor: 4.792
  2 in total

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