| Literature DB >> 33137198 |
Valentina Riva1, Anna Garbelli1, Federica Casiraghi1, Francesca Arena1, Claudia Immacolata Trivisani2, Assunta Gagliardi3, Luca Bini3, Martina Schroeder4, Antonio Maffia1, Simone Sabbioneda1, Giovanni Maga1.
Abstract
Removal of ribonucleotides (rNMPs) incorporated into the genome by the ribonucleotide excision repair (RER) is essential to avoid genetic instability. In eukaryotes, the RNaseH2 is the only known enzyme able to incise 5' of the rNMP, starting the RER process, which is subsequently carried out by replicative DNA polymerases (Pols) δ or ϵ, together with Flap endonuclease 1 (Fen-1) and DNA ligase 1. Here, we show that the DEAD-box RNA helicase DDX3X has RNaseH2-like activity and can support fully reconstituted in vitro RER reactions, not only with Pol δ but also with the repair Pols β and λ. Silencing of DDX3X causes accumulation of rNMPs in the cellular genome. These results support the existence of alternative RER pathways conferring high flexibility to human cells in responding to the threat posed by rNMPs incorporation.Entities:
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Year: 2020 PMID： 33137198 PMCID： PMC7672437 DOI： 10.1093/nar/gkaa948
Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971