Literature DB >> 33135675

Multi-crystal data collection using synchrotron radiation as exemplified with low-symmetry crystals of Dps.

Vladislav Kovalenko1, Alexander Popov2, Gianluca Santoni2, Natalia Loiko3, Ksenia Tereshkina1, Eduard Tereshkin1, Yurii Krupyanskii1.   

Abstract

Multi-crystal data collection using synchrotron radiation was successfully applied to determine the three-dimensional structure of a triclinic crystal form of Dps from Escherichia coli at 2.0 Å resolution. The final data set was obtained by combining 261 partial diffraction data sets measured from crystals with an average size of approximately 5 µm. The most important features of diffraction data measurement and processing for low-symmetry crystals are discussed.

Entities:  

Keywords:  clustering analysis; muti-crystal data collection; protein crystallography

Mesh：

Substances：

Year:  2020        PMID： 33135675      PMCID： PMC7605109          DOI： 10.1107/S2053230X20012571

Source DB:  PubMed          Journal:  Acta Crystallogr F Struct Biol Commun        ISSN： 2053-230X            Impact factor:   1.056


  26 in total

1.  Regulated phase transitions of bacterial chromatin: a non-enzymatic pathway for generic DNA protection.

Authors:  D Frenkiel-Krispin; S Levin-Zaidman; E Shimoni; S G Wolf; E J Wachtel; T Arad; S E Finkel; R Kolter; A Minsky
Journal:  EMBO J       Date:  2001-03-01       Impact factor: 11.598

2.  The application of hierarchical cluster analysis to the selection of isomorphous crystals.

Authors:  Rita Giordano; Ricardo M F Leal; Gleb P Bourenkov; Sean McSweeney; Alexander N Popov
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2012-05-17

3.  The ID23-1 structural biology beamline at the ESRF.

Authors:  Didier Nurizzo; Trevor Mairs; Matias Guijarro; Vicente Rey; Jens Meyer; Pablo Fajardo; Joel Chavanne; Jean Claude Biasci; Sean McSweeney; Edward Mitchell
Journal:  J Synchrotron Radiat       Date:  2006-04-13       Impact factor: 2.616

4.  Application of an in vitro DNA protection assay to visualize stress mediation properties of the Dps protein.

Authors:  Vlad O Karas; Ilja Westerlaken; Anne S Meyer
Journal:  J Vis Exp       Date:  2013-05-31       Impact factor: 1.355

5.  Molecular replacement with MOLREP.

Authors:  Alexei Vagin; Alexei Teplyakov
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2009-12-21

6.  A procedure for the large-scale isolation of highly purified plasmid DNA using alkaline extraction and binding to glass powder.

Authors:  M A Marko; R Chipperfield; H C Birnboim
Journal:  Anal Biochem       Date:  1982-04       Impact factor: 3.365

7.  Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity.

Authors:  T A Azam; A Ishihama
Journal:  J Biol Chem       Date:  1999-11-12       Impact factor: 5.157

8.  Optimization of data collection taking radiation damage into account.

Authors:  Gleb P Bourenkov; Alexander N Popov
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2010-03-24

9.  REFMAC5 for the refinement of macromolecular crystal structures.

Authors:  Garib N Murshudov; Pavol Skubák; Andrey A Lebedev; Navraj S Pannu; Roberto A Steiner; Robert A Nicholls; Martyn D Winn; Fei Long; Alexei A Vagin
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2011-03-18

Review 10.  Role of Dps (DNA-binding proteins from starved cells) aggregation on DNA.

Authors:  Emilia Chiancone; Pierpaolo Ceci
Journal:  Front Biosci (Landmark Ed)       Date:  2010-01-01
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