Literature DB >> 2510171

Physical mapping of the Myxococcus xanthus genome by random cloning in yeast artificial chromosomes.

A Kuspa1, D Vollrath, Y Cheng, D Kaiser.   

Abstract

Random segments of Myxococcus xanthus DNA were cloned in yeast artificial chromosomes (YACs) to construct a physical map of the genome. EcoRI restriction maps of 409 YAC clones with inserts averaging 111 kilobase pairs (kb) were determined. Comparison to the map of a 300-kb region of M. xanthus obtained from clones in Escherichia coli indicates that segments of DNA cloned in YACs are stably maintained in yeast and that their sequences accurately reflect the structure of the Myxococcus genome. The 409 YAC inserts were ordered within 60 map segments (contigs) by aligning their EcoRI restriction maps and by hybridization with 18 gene-specific DNA probes. These 60 map segments may represent the entire Myxococcus genome and could be used to organize its genetic information. This study illustrates the utility of YACs for cloning large segments of DNA and for reliable long-range genomic mapping.

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Year:  1989        PMID： 2510171      PMCID： PMC298401          DOI： 10.1073/pnas.86.22.8917

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  30 in total

1.  Toward a physical map of the genome of the nematode Caenorhabditis elegans.

Authors:  A Coulson; J Sulston; S Brenner; J Karn
Journal:  Proc Natl Acad Sci U S A       Date:  1986-10       Impact factor: 11.205

2.  Random-clone strategy for genomic restriction mapping in yeast.

Authors:  M V Olson; J E Dutchik; M Y Graham; G M Brodeur; C Helms; M Frank; M MacCollin; R Scheinman; T Frank
Journal:  Proc Natl Acad Sci U S A       Date:  1986-10       Impact factor: 11.205

3.  Electrophoretic separations of large DNA molecules by periodic inversion of the electric field.

Authors:  G F Carle; M Frank; M V Olson
Journal:  Science       Date:  1986-04-04       Impact factor: 47.728

4.  Use of recombination techniques to examine the structure of the csg locus of Myxococcus xanthus.

Authors:  L J Shimkets; S J Asher
Journal:  Mol Gen Genet       Date:  1988-01

5.  Physical mapping of large DNA by chromosome fragmentation.

Authors:  D Vollrath; R W Davis; C Connelly; P Hieter
Journal:  Proc Natl Acad Sci U S A       Date:  1988-08       Impact factor: 11.205

6.  Genome linking with yeast artificial chromosomes.

Authors:  A Coulson; R Waterston; J Kiff; J Sulston; Y Kohara
Journal:  Nature       Date:  1988-09-08       Impact factor: 49.962

7.  Cloning and complementation analysis of the "Frizzy" genes of Myxococcus xanthus.

Authors:  B D Blackhart; D R Zusman
Journal:  Mol Gen Genet       Date:  1985

8.  Yeast artificial chromosomes with 200- to 800-kilobase inserts of human DNA containing HLA, V kappa, 5S, and Xq24-Xq28 sequences.

Authors:  R D Little; G Porta; G F Carle; D Schlessinger; M D'Urso
Journal:  Proc Natl Acad Sci U S A       Date:  1989-03       Impact factor: 11.205

9.  Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors:  D C Schwartz; C R Cantor
Journal:  Cell       Date:  1984-05       Impact factor: 41.582

10.  Coliphage P1-mediated transduction of cloned DNA from Escherichia coli to Myxococcus xanthus: use for complementation and recombinational analyses.

Authors:  K A O'Connor; D R Zusman
Journal:  J Bacteriol       Date:  1983-07       Impact factor: 3.490
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  15 in total

1.  Amplification of large artificial chromosomes.

Authors:  D R Smith; A P Smyth; D T Moir
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

2.  Theoretical analysis of a physical mapping strategy using random single-copy landmarks.

Authors:  E Barillot; J Dausset; D Cohen
Journal:  Proc Natl Acad Sci U S A       Date:  1991-05-01       Impact factor: 11.205

3.  Chromosome map of Xanthomonas campestris pv. campestris 17 with locations of genes involved in xanthan gum synthesis and yellow pigmentation.

Authors:  Y H Tseng; K T Choy; C H Hung; N T Lin; J Y Liu; C H Lou; B Y Yang; F S Wen; S F Weng; J R Wu
Journal:  J Bacteriol       Date:  1999-01       Impact factor: 3.490

4.  Construction of a chromosome map for the phage group II Staphylococcus aureus Ps55.

Authors:  J P Bannantine; P A Pattee
Journal:  J Bacteriol       Date:  1996-12       Impact factor: 3.490

Review 5.  Physical mapping of bacterial genomes.

Authors:  M Fonstein; R Haselkorn
Journal:  J Bacteriol       Date:  1995-06       Impact factor: 3.490

6.  A physical map of the Myxococcus xanthus chromosome.

Authors:  Q He; H Chen; A Kuspa; Y Cheng; D Kaiser; L J Shimkets
Journal:  Proc Natl Acad Sci U S A       Date:  1994-09-27       Impact factor: 11.205

7.  Physical map of the Myxococcus xanthus chromosome.

Authors:  H W Chen; A Kuspa; I M Keseler; L J Shimkets
Journal:  J Bacteriol       Date:  1991-03       Impact factor: 3.490

8.  Search for MHC-associated genes in human: five new genes centromeric to HLA-DP with yet unknown functions.

Authors:  M Janatipour; Y Naumov; A Ando; K Sugimura; N Okamoto; K Tsuji; K Abe; H Inoko
Journal:  Immunogenetics       Date:  1992       Impact factor: 2.846

9.  An ordered collection of Bacillus subtilis DNA segments cloned in yeast artificial chromosomes.

Authors:  V Azevedo; E Alvarez; E Zumstein; G Damiani; V Sgaramella; S D Ehrlich; P Serror
Journal:  Proc Natl Acad Sci U S A       Date:  1993-07-01       Impact factor: 11.205

10.  Cloning whole bacterial genomes in yeast.

Authors:  Gwynedd A Benders; Vladimir N Noskov; Evgeniya A Denisova; Carole Lartigue; Daniel G Gibson; Nacyra Assad-Garcia; Ray-Yuan Chuang; William Carrera; Monzia Moodie; Mikkel A Algire; Quang Phan; Nina Alperovich; Sanjay Vashee; Chuck Merryman; J Craig Venter; Hamilton O Smith; John I Glass; Clyde A Hutchison
Journal:  Nucleic Acids Res       Date:  2010-03-07       Impact factor: 16.971
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