| Literature DB >> 1347950 |
J M Rossi1, D T Burke, J C Leung, D S Koos, H Chen, S M Tilghman.
Abstract
A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17.Entities:
Mesh：
Substances：
Year: 1992 PMID： 1347950 PMCID： PMC48677 DOI： 10.1073/pnas.89.6.2456
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205