| Literature DB >> 33098807 |
Finn J Hawkins1, Shingo Suzuki2, Mary Lou Beermann3, Cristina Barillà2, Ruobing Wang4, Carlos Villacorta-Martin3, Andrew Berical1, J C Jean3, Jake Le Suer1, Taylor Matte3, Chantelle Simone-Roach4, Yang Tang5, Thorsten M Schlaeger6, Ana M Crane2, Nadine Matthias2, Sarah X L Huang2, Scott H Randell7, Joshua Wu8, Jason R Spence9, Gianni Carraro10, Barry R Stripp10, Andras Rab11, Eric J Sorsher11, Amjad Horani12, Steven L Brody12, Brian R Davis13, Darrell N Kotton14.
Abstract
The derivation of tissue-specific stem cells from human induced pluripotent stem cells (iPSCs) would have broad reaching implications for regenerative medicine. Here, we report the directed differentiation of human iPSCs into airway basal cells ("iBCs"), a population resembling the stem cell of the airway epithelium. Using a dual fluorescent reporter system (NKX2-1GFP;TP63tdTomato), we track and purify these cells as they first emerge as developmentally immature NKX2-1GFP+ lung progenitors and subsequently augment a TP63 program during proximal airway epithelial patterning. In response to primary basal cell medium, NKX2-1GFP+/TP63tdTomato+ cells display the molecular and functional phenotype of airway basal cells, including the capacity to self-renew or undergo multi-lineage differentiation in vitro and in tracheal xenografts in vivo. iBCs and their differentiated progeny model perturbations that characterize acquired and genetic airway diseases, including the mucus metaplasia of asthma, chloride channel dysfunction of cystic fibrosis, and ciliary defects of primary ciliary dyskinesia.Entities:
Keywords: airway regeneration; basal cells; directed differentiation; induced pluripotent stem cells
Mesh：
Year: 2020 PMID： 33098807 PMCID： PMC7796997 DOI： 10.1016/j.stem.2020.09.017
Source DB: PubMed Journal: Cell Stem Cell ISSN： 1875-9777 Impact factor: 24.633