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Literature DB >> 33094750

Site-specific chemical fatty-acylation for gain-of-function analysis of protein S-palmitoylation in live cells.

Yumeng Li¹, Shushu Wang, Yanchi Chen, Manjia Li, Xiaoshu Dong, Howard C Hang, Tao Peng.

Abstract

Protein S-palmitoylation, or S-fatty-acylation, regulates many fundamental cellular processes in eukaryotes. Herein, we present a chemical fatty-acylation approach that involves site-specific incorporation of cycloalkyne-containing unnatural amino acids and subsequent bioorthogonal reactions with fatty-acyl tetrazines to install fatty-acylation mimics at target protein sites, allowing gain-of-function analysis of S-palmitoylation in live cells.

Entities: Chemical

Mesh：

Substances：

Year: 2020 PMID： 33094750 PMCID： PMC8177487 DOI： 10.1039/d0cc06073a

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

23 in total

1. Inhibiting the palmitoylation/depalmitoylation cycle selectively reduces the growth of hematopoietic cells expressing oncogenic Nras.

Authors: Jin Xu; Christian Hedberg; Frank J Dekker; Qing Li; Kevin M Haigis; Eugene Hwang; Herbert Waldmann; Kevin Shannon
Journal: Blood Date: 2011-12-05 Impact factor: 22.113

Site-specific chemical fatty-acylation for gain-of-function analysis of protein S-palmitoylation in live cells.

1. Inhibiting the palmitoylation/depalmitoylation cycle selectively reduces the growth of hematopoietic cells expressing oncogenic Nras.

2. Biochemistry. Mimicking posttranslational modifications of proteins.

3. Synthesis and evaluation of a series of 1,2,4,5-tetrazines for bioorthogonal conjugation.

4. Global analysis of protein palmitoylation in yeast.

5. An acylation cycle regulates localization and activity of palmitoylated Ras isoforms.

6. Labeling proteins on live mammalian cells using click chemistry.

Review 7. The molecular era of protein S-acylation: spotlight on structure, mechanisms, and dynamics.

8. A bioorthogonal chemistry strategy for probing protein lipidation in live cells.

Review 9. Emerging Roles of DHHC-mediated Protein S-palmitoylation in Physiological and Pathophysiological Context.

Review 10. Expanding and reprogramming the genetic code.

1. Development of mammalian cell logic gates controlled by unnatural amino acids.

Review 2. Chemical approaches for investigating site-specific protein S-fatty acylation.

Review 3. Protein cysteine palmitoylation in immunity and inflammation.

Review 4. Regulation of Dynamic Protein S-Acylation.

5. Site-Specific Lipidation Enhances IFITM3 Membrane Interactions and Antiviral Activity.

Review 6. Protein S-palmitoylation in immunity.

Review 7. Palmitoylation in Crohn's disease: Current status and future directions.