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Literature DB >> 3301525

High frequency generalized transduction by miniMu plasmid phage.

B M Wang, L Liu, E A Groisman, M J Casadaban, C M Berg.

Abstract

Deletion derivatives of phage Mu which replicate as multicopy plasmids, and also transpose and package like Mu, have been developed for the in vivo cloning of bacterial genes. We show here that these miniMu plasmid phage are also efficient at generalized transduction and that both in vivo cloning and generalized transduction of a given gene can be accomplished in a single experiment.

Entities: Species

Mesh：

Year: 1987 PMID： 3301525 PMCID： PMC1203130

Source DB: PubMed Journal: Genetics ISSN： 0016-6731 Impact factor: 4.562

7 in total

1. Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria.

Authors: W V Shaw
Journal: Methods Enzymol Date: 1975 Impact factor: 1.600

2. Transposition derivatives of an Hfr strain of Escherichia coli K-12.

Authors: C M Berg; R Curtiss
Journal: Genetics Date: 1967-07 Impact factor: 4.562

3. Mini-Mu-duction as a test for genetic complementation in Escherichia coli.

Authors: J E Cronan
Journal: J Bacteriol Date: 1984-04 Impact factor: 3.490

4. In vivo DNA cloning and adjacent gene fusing with a mini-Mu-lac bacteriophage containing a plasmid replicon.

Authors: E A Groisman; B A Castilho; M J Casadaban
Journal: Proc Natl Acad Sci U S A Date: 1984-03 Impact factor: 11.205

5. Analysis of an avtA::Mu d1(Ap lac) mutant: metabolic role of transaminase C.

Authors: W A Whalen; C M Berg
Journal: J Bacteriol Date: 1982-05 Impact factor: 3.490

6. beta-Chloro-L-alanine inhibition of the Escherichia coli alanine-valine transaminase.

Authors: W A Whalen; M D Wang; C M Berg
Journal: J Bacteriol Date: 1985-12 Impact factor: 3.490

7. Infection of Salmonella typhimurium with coliphage Mu d1 (Apr lac): construction of pyr::lac gene fusions.

Authors: L N Csonka; M M Howe; J L Ingraham; L S Pierson; C L Turnbough
Journal: J Bacteriol Date: 1981-01 Impact factor: 3.490

7 in total

8 in total

1. Protein ProQ influences osmotic activation of compatible solute transporter ProP in Escherichia coli K-12.

Authors: H J Kunte; R A Crane; D E Culham; D Richmond; J M Wood
Journal: J Bacteriol Date: 1999-03 Impact factor: 3.490

2. Rapid sequencing of cloned DNA using a transposon for bidirectional priming: sequence of the Escherichia coli K-12 avtA gene.

Authors: L Liu; W Whalen; A Das; C M Berg
Journal: Nucleic Acids Res Date: 1987-11-25 Impact factor: 16.971

3. A functional leuABCD operon is required for leucine synthesis by the tyrosine-repressible transaminase in Escherichia coli K-12.

Authors: N B Vartak; L Liu; B M Wang; C M Berg
Journal: J Bacteriol Date: 1991-06 Impact factor: 3.490

High frequency generalized transduction by miniMu plasmid phage.

1. Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria.

2. Transposition derivatives of an Hfr strain of Escherichia coli K-12.

3. Mini-Mu-duction as a test for genetic complementation in Escherichia coli.

4. In vivo DNA cloning and adjacent gene fusing with a mini-Mu-lac bacteriophage containing a plasmid replicon.

5. Analysis of an avtA::Mu d1(Ap lac) mutant: metabolic role of transaminase C.

6. beta-Chloro-L-alanine inhibition of the Escherichia coli alanine-valine transaminase.

7. Infection of Salmonella typhimurium with coliphage Mu d1 (Apr lac): construction of pyr::lac gene fusions.

1. Protein ProQ influences osmotic activation of compatible solute transporter ProP in Escherichia coli K-12.

2. Rapid sequencing of cloned DNA using a transposon for bidirectional priming: sequence of the Escherichia coli K-12 avtA gene.

3. A functional leuABCD operon is required for leucine synthesis by the tyrosine-repressible transaminase in Escherichia coli K-12.

4. Cloning and characterization of the Escherichia coli K-12 alanine-valine transaminase (avtA) gene.

5. Rapid identification of bacterial genes that are lethal when cloned on multicopy plasmids.

6. Tn5tac1, a derivative of transposon Tn5 that generates conditional mutations.

7. In vivo cloning of Pseudomonas aeruginosa genes with mini-D3112 transposable bacteriophage.

8. Cloning of genes that suppress an Escherichia coli K-12 alanine auxotroph when present in multicopy plasmids.