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Literature DB >> 3275630

Rapid identification of bacterial genes that are lethal when cloned on multicopy plasmids.

Abstract

A procedure to identify genes that are lethal when cloned on multicopy plasmids was developed. It depends on the ability of mini-Mu plasmid elements to be used for both in vivo cloning and generalized transduction of enterobacterial genes. The feasibility of this procedure was demonstrated by using the tetA gene of Tn10, which is lethal when in multiple copies in the presence of 25 micrograms of tetracycline per ml.

Entities: Chemical Species

Mesh：

Year: 1988 PMID： 3275630 PMCID： PMC210670 DOI： 10.1128/jb.170.1.468-470.1988

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

14 in total

1. Fusion of the Escherichia coli lac genes to the ara promoter: a general technique using bacteriophage Mu-1 insertions.

Authors: M J Casadaban
Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

2. Generation of miniplasmids from copy number mutants of the R plasmid NR1.

Authors: D P Taylor; J Greenberg; R H Rownd
Journal: J Bacteriol Date: 1977-12 Impact factor: 3.490

3. Positive selection for loss of tetracycline resistance.

Authors: B R Bochner; H C Huang; G L Schieven; B N Ames
Journal: J Bacteriol Date: 1980-08 Impact factor: 3.490

4. Nucleotide sequence of the Tn10 encoded tetracycline resistance gene.

Authors: W Hillen; K Schollmeier
Journal: Nucleic Acids Res Date: 1983-01-25 Impact factor: 16.971

5. In vivo DNA cloning and adjacent gene fusing with a mini-Mu-lac bacteriophage containing a plasmid replicon.

Authors: E A Groisman; B A Castilho; M J Casadaban
Journal: Proc Natl Acad Sci U S A Date: 1984-03 Impact factor: 11.205

6. Analysis of an avtA::Mu d1(Ap lac) mutant: metabolic role of transaminase C.

Authors: W A Whalen; C M Berg
Journal: J Bacteriol Date: 1982-05 Impact factor: 3.490

7. Genetic and biochemical analyses of pantothenate biosynthesis in Escherichia coli and Salmonella typhimurium.

Authors: J E Cronan; K J Littel; S Jackowski
Journal: J Bacteriol Date: 1982-03 Impact factor: 3.490

8. Mutations in multicopy Tn10 tet plasmids that confer resistance to inhibitory effects of inducers of tet gene expression.

Authors: H S Moyed; K P Bertrand
Journal: J Bacteriol Date: 1983-08 Impact factor: 3.490

9. Multicopy Tn10 tet plasmids confer sensitivity to induction of tet gene expression.

Authors: H S Moyed; T T Nguyen; K P Bertrand
Journal: J Bacteriol Date: 1983-08 Impact factor: 3.490

10. Genetic organization of transposon Tn10.

Authors: T J Foster; M A Davis; D E Roberts; K Takeshita; N Kleckner
Journal: Cell Date: 1981-01 Impact factor: 41.582

4 in total

1. A functional leuABCD operon is required for leucine synthesis by the tyrosine-repressible transaminase in Escherichia coli K-12.

Authors: N B Vartak; L Liu; B M Wang; C M Berg
Journal: J Bacteriol Date: 1991-06 Impact factor: 3.490