| Literature DB >> 32943808 |
Francis W L Esmonde-White1, Matthew V Schulmerich1, Karen A Esmonde-White2, Michael D Morris1.
Abstract
Raman spectroscopy of bone is complicated by fluorescence background and spectral contributions from other tissues. Full utilization of Raman spectroscopy in bone studies requires rapid and accurate calibration and preprocessing methods. We have taken a step-wise approach to optimize and automate calibrations, preprocessing and background correction. Improvements to manual spike removal, white light correction, software image rotation and slit image curvature correction are described. Our approach is concisely described with a minimum of mathematical detail.Entities:
Keywords: Raman Spectroscopy; background correction; bone; dichroic filter spectra; fluorescence removal; image rotation; musculoskeletal tissue; preprocessing; spike removal
Year: 2009 PMID: 32943808 PMCID: PMC7493803 DOI: 10.1117/12.809436
Source DB: PubMed Journal: Proc SPIE Int Soc Opt Eng ISSN: 0277-786X