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Literature DB >> 329276

Experimental evidence for kinetic proofreading in the aminoacylation of tRNA by synthetase.

Abstract

The enzymatic aminoacylation of tRNA can be viewed as a means of proofreading either the amino acid or the tRNA or both. We have conducted further experimental tests of kinetic proofreading in discriminating between cognate and noncognate amino acids and tRNAs as follows: (formula: see text). In cases (i) and (ii) the amino acids are proofread, in cases (iii) and (iv) the tRNA is proofread, and in case (v), both the amino acid and the tRNA are proofread. ATP consumed per acylation was 400, 1.5, 40, 25, and 1000, respectively. High ATP/aminoacylation ratios are diagnostic for kinetic proofreading.

Entities: Chemical Gene

Mesh：

Substances：

Year: 1977 PMID： 329276 PMCID： PMC432146 DOI： 10.1073/pnas.74.6.2246

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

44 in total

1. The specificity of protein biosynthesis.

Authors: M VAUGHAN; D STEINBERG
Journal: Adv Protein Chem Date: 1959

2. Proofreading of the codon-anticodon interaction on ribosomes.

Authors: R C Thompson; P J Stone
Journal: Proc Natl Acad Sci U S A Date: 1977-01 Impact factor: 11.205

3. Incorrect aminoacylations involving tRNAs or valyl-tRNA synthetase from Bacillus stearothermophilus.

Authors: R Giegé; D Kern; J P Ebel; H Grosjean; S de Henau; H Chantrenne
Journal: Eur J Biochem Date: 1974-06-15

4. Correction of aminoacylation errors: evidence for a non significant role of the aminoacyl-tRNA synthetase catalysed deacylation of aminoacyl-tRNAs.

Authors: J Bonnet; J P Ebel
Journal: FEBS Lett Date: 1974-03-01 Impact factor: 4.124

5. Structural requirements for recognition of Escherichia coli initiator and non-initiator transfer ribonucleic acids by bacterial T factor.

Authors: L H Schulman; H Pelka; R M Sundari
Journal: J Biol Chem Date: 1974-11-25 Impact factor: 5.157

6. Recognition of tRNA by isoleucyl-tRNA synthetase. Effect of substrates on the dynamics of tRNA-enzyme interaction.

Authors: M Yarus; P Berg
Journal: J Mol Biol Date: 1969-06-14 Impact factor: 5.469

7. Interpretation of tRNA-mischarging kinetics.

Authors: A Dietrich; D Kern; J Bonnet; R Giegé; J P Ebel
Journal: Eur J Biochem Date: 1976-11-01

8. Energy metabolism of Saccharomyces cerevisiae discrepancy between ATP balance and known metabolic functions.

Authors: R Lagunas
Journal: Biochim Biophys Acta Date: 1976-09-13

9. Hydrolytic action of aminoacyl-tRNA synthetases from baker's yeast: "chemical proofreading" preventing acylation of tRNA(I1e) with misactivated valine.

Authors: F von der Haar; F Cramer
Journal: Biochemistry Date: 1976-09-07 Impact factor: 3.162

8. Preferential hydrophobic interactions are responsible for a preference of D-amino acids in the aminoacylation of 5'-AMP with hydrophobic amino acids.

Authors: J C Lacey; N S Wickramasinghe; R S Sabatini
Journal: Experientia Date: 1992-04-15

9. The energy relay: a proofreading scheme based on dynamic cooperativity and lacking all characteristic symptoms of kinetic proofreading in DNA replication and protein synthesis.

Authors: J J Hopfield
Journal: Proc Natl Acad Sci U S A Date: 1980-09 Impact factor: 11.205

10. In vitro protein synthesis using D-amino acids and its evolutionary significance.

Authors: M Isoyama; H Ohoka; H Kikuchi; A Shimada; S Yuasa
Journal: Orig Life Date: 1984

Experimental evidence for kinetic proofreading in the aminoacylation of tRNA by synthetase.

1. The specificity of protein biosynthesis.

2. Proofreading of the codon-anticodon interaction on ribosomes.

3. Incorrect aminoacylations involving tRNAs or valyl-tRNA synthetase from Bacillus stearothermophilus.

4. Correction of aminoacylation errors: evidence for a non significant role of the aminoacyl-tRNA synthetase catalysed deacylation of aminoacyl-tRNAs.

5. Structural requirements for recognition of Escherichia coli initiator and non-initiator transfer ribonucleic acids by bacterial T factor.

6. Recognition of tRNA by isoleucyl-tRNA synthetase. Effect of substrates on the dynamics of tRNA-enzyme interaction.

7. Interpretation of tRNA-mischarging kinetics.

8. Energy metabolism of Saccharomyces cerevisiae discrepancy between ATP balance and known metabolic functions.

9. Hydrolytic action of aminoacyl-tRNA synthetases from baker's yeast: "chemical proofreading" preventing acylation of tRNA(I1e) with misactivated valine.

10. A soluble ATP-dependent proteolytic system responsible for the degradation of abnormal proteins in reticulocytes.

Review 1. Editing of errors in selection of amino acids for protein synthesis.

2. Speed, dissipation, and error in kinetic proofreading.

3. Aminoacyl transfer rate dictates choice of editing pathway in threonyl-tRNA synthetase.

4. Kinetic discrimination of tRNA identity by the conserved motif 2 loop of a class II aminoacyl-tRNA synthetase.

5. Kinetic proofreading in chromatin remodeling: the case of ISWI/ACF.

6. Proofreading in vivo: editing of homocysteine by methionyl-tRNA synthetase in Escherichia coli.

7. Proofreading through spatial gradients.

8. Preferential hydrophobic interactions are responsible for a preference of D-amino acids in the aminoacylation of 5'-AMP with hydrophobic amino acids.

9. The energy relay: a proofreading scheme based on dynamic cooperativity and lacking all characteristic symptoms of kinetic proofreading in DNA replication and protein synthesis.

10. In vitro protein synthesis using D-amino acids and its evolutionary significance.