Carolyn M McClaskey1, Clarisse H Panganiban2, Kenyaria V Noble3, James W Dias4, Hainan Lang5, Kelly C Harris6. 1. Department of Otolaryngology, Head & Neck Surgery, Medical University of South Carolina, 135 Rutledge Ave, MSC 550, Charleston, SC, 29425, United States. Electronic address: mcclaske@musc.edu. 2. Department of Pathology and Laboratory Medicine, Medical University of South Carolina, 171 Ashley Avenue, MSC 908, Charleston, SC, 29425, United States. Electronic address: clarisse.panganiban@kcl.ac.uk. 3. Department of Pathology and Laboratory Medicine, Medical University of South Carolina, 171 Ashley Avenue, MSC 908, Charleston, SC, 29425, United States. Electronic address: noble@musc.edu. 4. Department of Otolaryngology, Head & Neck Surgery, Medical University of South Carolina, 135 Rutledge Ave, MSC 550, Charleston, SC, 29425, United States. Electronic address: diasj@musc.edu. 5. Department of Pathology and Laboratory Medicine, Medical University of South Carolina, 171 Ashley Avenue, MSC 908, Charleston, SC, 29425, United States. Electronic address: langh@musc.edu. 6. Department of Otolaryngology, Head & Neck Surgery, Medical University of South Carolina, 135 Rutledge Ave, MSC 550, Charleston, SC, 29425, United States. Electronic address: harriskc@musc.edu.
Abstract
BACKGROUND: The auditory brainstem response (ABR), specifically wave I, is widely used to noninvasively measure auditory nerve (AN) function. Recent work in humans has introduced novel electrocochleographic measures to comprehensively characterize AN function that emphasize suprathreshold processing and estimate neural synchrony. NEW METHOD: This study establishes new tools for evaluating AN function in vivo in adult mice using tone-evoked ABRs obtained from young-adult CBA/CaJ mice, adapting the approach previously introduced in humans. Six metrics are obtained from ABR wave I at suprathreshold stimulus levels. RESULTS: Change-point analyses show that the metrics' rate of change with stimulus level differs between moderate and high suprathreshold levels, suggesting that this approach can potentially characterize the presence of heterogeneous AN fiber types. COMPARISON WITH EXISTING METHODS: Traditional ABR approaches focus on response thresholds and averaged amplitudes/latencies. In contrast, our multi-metric approach, which uses single-trial data and suprathreshold stimuli, provides novel information and identifies evidence of neural synchrony deficits and changes in the heterogeneity of AN fibers underlying AN behavior. CONCLUSION: The techniques reported here provide a novel tool to assess changes in AN function in vivo in a commonly used animal model. A benchmark of most current hearing research is the transition from animal to human studies. Here we established a translational objective approach, applying methods that were first developed in humans to animals. This approach enables researchers to identify changes in AN function arising from the animal models with well-characterized pathology, and predict similar pathological changes in human AN dysfunction and hearing loss.
BACKGROUND: The auditory brainstem response (ABR), specifically wave I, is widely used to noninvasively measure auditory nerve (AN) function. Recent work in humans has introduced novel electrocochleographic measures to comprehensively characterize AN function that emphasize suprathreshold processing and estimate neural synchrony. NEW METHOD: This study establishes new tools for evaluating AN function in vivo in adult mice using tone-evoked ABRs obtained from young-adult CBA/CaJ mice, adapting the approach previously introduced in humans. Six metrics are obtained from ABR wave I at suprathreshold stimulus levels. RESULTS: Change-point analyses show that the metrics' rate of change with stimulus level differs between moderate and high suprathreshold levels, suggesting that this approach can potentially characterize the presence of heterogeneous AN fiber types. COMPARISON WITH EXISTING METHODS: Traditional ABR approaches focus on response thresholds and averaged amplitudes/latencies. In contrast, our multi-metric approach, which uses single-trial data and suprathreshold stimuli, provides novel information and identifies evidence of neural synchrony deficits and changes in the heterogeneity of AN fibers underlying AN behavior. CONCLUSION: The techniques reported here provide a novel tool to assess changes in AN function in vivo in a commonly used animal model. A benchmark of most current hearing research is the transition from animal to human studies. Here we established a translational objective approach, applying methods that were first developed in humans to animals. This approach enables researchers to identify changes in AN function arising from the animal models with well-characterized pathology, and predict similar pathological changes in human AN dysfunction and hearing loss.
Authors: Clarisse H Panganiban; Jeremy L Barth; Lama Darbelli; Yazhi Xing; Jianning Zhang; Hui Li; Kenyaria V Noble; Ting Liu; LaShardai N Brown; Bradley A Schulte; Stéphane Richard; Hainan Lang Journal: J Neurosci Date: 2018-02-06 Impact factor: 6.167
Authors: Clarisse H Panganiban; Jeremy L Barth; Junying Tan; Kenyaria V Noble; Carolyn M McClaskey; Blake A Howard; Shabih H Jafri; James W Dias; Kelly C Harris; Hainan Lang Journal: Glia Date: 2021-12-29 Impact factor: 8.073
Authors: Kelly C Harris; Jayne B Ahlstrom; James W Dias; Lilyana B Kerouac; Carolyn M McClaskey; Judy R Dubno; Mark A Eckert Journal: J Neurosci Date: 2021-11-09 Impact factor: 6.709
Authors: Jeffrey A Rumschlag; Carolyn M McClaskey; James W Dias; Lilyana B Kerouac; Kenyaria V Noble; Clarisse Panganiban; Hainan Lang; Kelly C Harris Journal: Neurobiol Aging Date: 2022-03-25 Impact factor: 5.133