Multiple angle digital holography for the shape measurement of the unpainted tympanic membrane.

The shape of the tympanic membrane (TM) plays an important role in sound transmission through the ear for hearing. Previously we developed a high-speed holographic system employing a tunable wavelength laser for rapid TM shape measurement. However, the tunable laser illumination was not sufficient to measure the shape of the unpainted TM due to the semi-transparency of the TM and short exposure time of the camera. This paper presents a new multiple angle illumination technique that allows us to use a higher power single wavelength laser to perform shape measurements on the unpainted TM. Accuracy of the new method is demonstrated by a measure of a step gauge provided by the National Institute of Standards and Technology. We successfully applied the new shape measurement method on a fresh postmortem human TM without any paint.