Jesús Z Villarreal1,2, J Pérez-Anker3, Luis F Quintana4,5,6, A García-Herrera7,8, S Puig3,9, G Pellacani10, M Solé7,8, J Malvehy3,9. 1. Nephrology and Renal Transplantation Department, Hospital Clínic de Barcelona, Universitat de Barcelona, Barcelona, Spain. 2. Institut d'Investigacions Biomediques August Pi I Sunyer (IDIBAPS), Barcelona, Spain. 3. Dermatology Department, Melanoma Unit, Hospital Clínic de Barcelona, Institut d'Investigacions Biomediques August Pi I Sunyer (IDIBAPS), Universitat de Barcelona, Barcelona, Spain. 4. Nephrology and Renal Transplantation Department, Hospital Clínic de Barcelona, Universitat de Barcelona, Barcelona, Spain. lfquinta@clinic.cat. 5. Institut d'Investigacions Biomediques August Pi I Sunyer (IDIBAPS), Barcelona, Spain. lfquinta@clinic.cat. 6. Centro de Referencia en Enfermedad Glomerular Compleja del Sistema Nacional de Salud (CSUR), Barcelona, Spain. lfquinta@clinic.cat. 7. Pathology Department, Hospital Clínic de Barcelona, Universitat de Barcelona, Barcelona, Spain. 8. Centro de Referencia en Enfermedad Glomerular Compleja del Sistema Nacional de Salud (CSUR), Barcelona, Spain. 9. Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Barcelona, Spain. 10. Department of Dermatology, University of Modena and Reggio Emilia, Modena, Italy.
Abstract
BACKGROUND: Ex vivo confocal microscopy is a technique for tissue examination, which generates images of fresh samples with an optical resolution comparable to those obtained by conventional pathology. The objective of this study was to evaluate the feasibility of using ex vivo confocal microscopy in fusion mode (reflectance and fluorescence) and the H&E-like digital staining that is obtained for the analysis of non-neoplastic kidney biopsies. METHODS: Twenty-four renal samples acquired from autopsies were scanned in a 4th generation ex vivo confocal microscopy device. The imaging process was completed in an average of three minutes. RESULTS: Confocal images correlated very well to the corresponding conventional histological sections, both in normal tissue and in chronic lesions (glomerulosclerosis, fibrosis and tubular atrophy). The ex vivo confocal microscopy protocol did not add artifacts to the sample for the ulterior study with light microscopy, nor to the histochemical or immunohistochemical studies. CONCLUSION: The ease and speed of grayscale and fluorescence image acquisition, together with the quality of the H&E-like digitally stained images obtained with this approach, suggest that this technique shows promise for use in clinical nephrology and renal transplantation.
BACKGROUND: Ex vivo confocal microscopy is a technique for tissue examination, which generates images of fresh samples with an optical resolution comparable to those obtained by conventional pathology. The objective of this study was to evaluate the feasibility of using ex vivo confocal microscopy in fusion mode (reflectance and fluorescence) and the H&E-like digital staining that is obtained for the analysis of non-neoplastic kidney biopsies. METHODS: Twenty-four renal samples acquired from autopsies were scanned in a 4th generation ex vivo confocal microscopy device. The imaging process was completed in an average of three minutes. RESULTS: Confocal images correlated very well to the corresponding conventional histological sections, both in normal tissue and in chronic lesions (glomerulosclerosis, fibrosis and tubular atrophy). The ex vivo confocal microscopy protocol did not add artifacts to the sample for the ulterior study with light microscopy, nor to the histochemical or immunohistochemical studies. CONCLUSION: The ease and speed of grayscale and fluorescence image acquisition, together with the quality of the H&E-like digitally stained images obtained with this approach, suggest that this technique shows promise for use in clinical nephrology and renal transplantation.
Entities:
Keywords:
Ex vivo confocal microscopy; Fluorescence confocal microscopy; Fusion confocal microscopy; Reflectance confocal microscopy; Renal biopsy
Authors: J Pérez-Anker; S Ribero; O Yélamos; A García-Herrera; L Alos; B Alejo; M Combalia; D Moreno-Ramírez; J Malvehy; S Puig Journal: Br J Dermatol Date: 2019-10-27 Impact factor: 9.302
Authors: Martin-Walter Welker; Nina Weiler; Wolf Otto Bechstein; Eva Herrmann; Christoph Betz; Mark Schöffauer; Stefan Zeuzem; Christoph Sarrazin; Kerstin Amann; Oliver Jung Journal: J Nephrol Date: 2018-06-26 Impact factor: 3.902
Authors: Marco Fiorentino; Davide Bolignano; Vladimir Tesar; Anna Pisano; Wim Van Biesen; Graziella D'Arrigo; Giovanni Tripepi; Loreto Gesualdo Journal: Am J Nephrol Date: 2016-02-05 Impact factor: 3.754
Authors: Stefano Puliatti; Ahmed Eissa; Enrico Checcucci; Pietro Piazza; Marco Amato; Stefania Ferretti; Simone Scarcella; Juan Gomez Rivas; Mark Taratkin; Josè Marenco; Ines Belenchon Rivero; Karl-Friedrich Kowalewski; Giovanni Cacciamani; Ahmed El-Sherbiny; Ahmed Zoeir; Abdelhamid M El-Bahnasy; Ruben De Groote; Alexandre Mottrie; Salvatore Micali Journal: Asian J Urol Date: 2022-06-01