Stefano Puliatti1, Laura Bertoni2, Giacomo M Pirola1, Paola Azzoni2, Luigi Bevilacqua1, Ahmed Eissa1,3, Ahmed Elsherbiny1,3, Maria C Sighinolfi1, Johanna Chester2, Shaniko Kaleci2, Bernardo Rocco1, Salvatore Micali1, Ilaria Bagni4, Luca Reggiani Bonetti4, Antonino Maiorana4, Josep Malvehy5, Caterina Longo6,7, Rodolfo Montironi8, Giampaolo Bianchi1,2, Giovanni Pellacani2,7. 1. Department of Urology, University of Modena and Reggio Emilia, Modena, Italy. 2. Department of Surgical, Medical, Dental and Morphological Sciences with Interest Transplant, Oncological and Regenerative Medicine, University of Modena and Reggio Emilia, Modena, Italy. 3. Urology Department, Faculty of Medicine, Tanta University, Tanta, Egypt. 4. Department of Pathology, Ospedale Policlinico e Nuovo Ospedale Civile S. Agostino Estense Modena, University of Modena and Reggio Emilia, Modena, Italy. 5. Melanoma Unit, Dermatology Department, Hospital Clinic, Barcelona, Spain. 6. Azienda Unit Sanitaria Locale - IRCCS di Reggio Emilia, Centro Oncologico ad Alta Tecnologia Diagnostica- Dermatologia, Reggio Emilia Modena, Italy. 7. Department of Dermatology, University of Modena and Reggio Emilia, Modena, Italy. 8. Department of Pathological Anatomy, Polytechnic University of the Marche Region, School of Medicine, United Hospitals, Ancona, Italy.
Abstract
OBJECTIVE: To report the first application of ex vivo fluorescence confocal microscopy (FCM) - a novel optical technology that is capable of providing fast microscopic imaging of unfixed tissue specimens- in the urological field assessing its diagnostic accuracy for non neoplastic and cancerous prostate tissue (prostatic adenocarcinoma) compared to the 'gold standard' histopathological diagnoses. PATIENTS AND METHODS: In all, 89 specimens from 13 patients with clinically localised prostate cancer were enrolled into the study. All patients underwent robot-assisted laparoscopic radical prostatectomy with fresh prostatic tissue biopsies taken at the end of each intervention using an 18-G biopsy punch. Specimens were randomly assigned to the three collaborating pathologists for evaluation. Intra- and inter-observer agreement was tested by the means of Cohen's κ. The diagnostic performance was evaluated on receiver operating characteristic curve analysis. RESULTS: The overall diagnostic agreement between FCM and histopathological diagnoses was substantial with a 91% correct diagnosis (κ = 0.75) and an area under the curve of 0.884 (95% confidence interval 0.840-0.920), 83.33% sensitivity, and 93.53% specificity. CONCLUSION: FCM seems to be a promising tool for enhanced specimens' reporting performance, given its simple application and very rapid microscopic image generation (<5 min/specimen). This technique may potentially be used for intraoperative pathological specimens' analysis.
OBJECTIVE: To report the first application of ex vivo fluorescence confocal microscopy (FCM) - a novel optical technology that is capable of providing fast microscopic imaging of unfixed tissue specimens- in the urological field assessing its diagnostic accuracy for non neoplastic and cancerous prostate tissue (prostatic adenocarcinoma) compared to the 'gold standard' histopathological diagnoses. PATIENTS AND METHODS: In all, 89 specimens from 13 patients with clinically localised prostate cancer were enrolled into the study. All patients underwent robot-assisted laparoscopic radical prostatectomy with fresh prostatic tissue biopsies taken at the end of each intervention using an 18-G biopsy punch. Specimens were randomly assigned to the three collaborating pathologists for evaluation. Intra- and inter-observer agreement was tested by the means of Cohen's κ. The diagnostic performance was evaluated on receiver operating characteristic curve analysis. RESULTS: The overall diagnostic agreement between FCM and histopathological diagnoses was substantial with a 91% correct diagnosis (κ = 0.75) and an area under the curve of 0.884 (95% confidence interval 0.840-0.920), 83.33% sensitivity, and 93.53% specificity. CONCLUSION:FCM seems to be a promising tool for enhanced specimens' reporting performance, given its simple application and very rapid microscopic image generation (<5 min/specimen). This technique may potentially be used for intraoperative pathological specimens' analysis.
Authors: Jesús Z Villarreal; J Pérez-Anker; Luis F Quintana; A García-Herrera; S Puig; G Pellacani; M Solé; J Malvehy Journal: J Nephrol Date: 2020-09-02 Impact factor: 3.902
Authors: Kivanc Kose; Christi Alessi Fox; Anthony Rossi; Manu Jain; Miguel Cordova; Stephen W Dusza; Moira Ragazzi; Stefano Gardini; Elvira Moscarella; Alba Diaz; Ramon Pigem; Salvador Gonzalez; Antoni Bennassar; Cristina Carrera; Caterina Longo; Milind Rajadhyaksha; Kishwer S Nehal Journal: J Cutan Pathol Date: 2021-03-03 Impact factor: 1.587
Authors: Stefano Puliatti; Ahmed Eissa; Enrico Checcucci; Pietro Piazza; Marco Amato; Stefania Ferretti; Simone Scarcella; Juan Gomez Rivas; Mark Taratkin; Josè Marenco; Ines Belenchon Rivero; Karl-Friedrich Kowalewski; Giovanni Cacciamani; Ahmed El-Sherbiny; Ahmed Zoeir; Abdelhamid M El-Bahnasy; Ruben De Groote; Alexandre Mottrie; Salvatore Micali Journal: Asian J Urol Date: 2022-06-01
Authors: James G Fujimoto; Yue Sun; Lucas C Cahill; Seymour Rosen; Tadayuki Yoshitake; Yubo Wu; Linda York; Leo L Tsai; Boris Gershman Journal: Mod Pathol Date: 2021-11-02 Impact factor: 8.209