Gavriella-Zoi Vrakopoulou1, Konstantinos G Toutouzas1, Panagiotis Giannios2, Sotirios-Georgios Panousopoulos1, Charalampos Theodoropoulos1, Eugene Danas3, Aliki Liakea3, Apostolos E Papalois4,5, George Zografos1, Andreas Larentzakis6. 1. First Department of Propaedeutic Surgery, Hippocration General Athens Hospital, Athens Medical School, National and Kapodistrian University of Athens, Athens, Greece. 2. Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Barcelona, Spain. 3. First Department of Pathology, Athens Medical School, National and Kapodistrian University of Athens, Athens, Greece. 4. Experimental, Educational and Research Center ELPEN, Pikermi, Greece. 5. School of Medicine, European University Cyprus, Nicosia, Cyprus. 6. First Department of Propaedeutic Surgery, Hippocration General Athens Hospital, Athens Medical School, National and Kapodistrian University of Athens, Athens, Greece alarentz@med.uoa.gr.
Abstract
BACKGROUND: Healing is related to gastrointestinal anastomotic leak, which is a severe and common complication. This study aimed to investigate the feasibility and the impact of deserosalization on healing of jejuno-jejunal anastomoses in an animal model. MATERIALS AND METHODS: Seven swine underwent three types of side-to-side jejuno-jejunal anastomosis twice and survived seven days. Three different types of jejuno-jejunal side-to-side anastomoses were performed twice at 20-cm distance from each other in each animal: no serosa removal, one-sided, and two-sided serosa removal, respectively. Bursting pressure, tissue hydroxyproline concentration, and pathology scores were evaluated. RESULTS: Hydroxyproline tissue concentration was a mean±standard deviation of 0.37±0.09, 0.38±0.08, and 0.30±0.05 nmoI/ml respectively (p<0.05). Bursting pressure was a mean±standard deviation of 59.02±8.60, 73.20±11.09, and 100.01±7.49 mmHg, respectively (p<0.001). The histopathological assessment did not find any statistically significant differences. CONCLUSION: Deserosalization in jejuno-jejunal anastomosis was technically feasible and seemed to improve mechanical strength and collagen deposition in this experimental porcine model. Further investigation is warranted. Copyright
BACKGROUND: Healing is related to gastrointestinal anastomotic leak, which is a severe and common complication. This study aimed to investigate the feasibility and the impact of deserosalization on healing of jejuno-jejunal anastomoses in an animal model. MATERIALS AND METHODS: Seven swine underwent three types of side-to-side jejuno-jejunal anastomosis twice and survived seven days. Three different types of jejuno-jejunal side-to-side anastomoses were performed twice at 20-cm distance from each other in each animal: no serosa removal, one-sided, and two-sided serosa removal, respectively. Bursting pressure, tissue hydroxyproline concentration, and pathology scores were evaluated. RESULTS:Hydroxyproline tissue concentration was a mean±standard deviation of 0.37±0.09, 0.38±0.08, and 0.30±0.05 nmoI/ml respectively (p<0.05). Bursting pressure was a mean±standard deviation of 59.02±8.60, 73.20±11.09, and 100.01±7.49 mmHg, respectively (p<0.001). The histopathological assessment did not find any statistically significant differences. CONCLUSION: Deserosalization in jejuno-jejunal anastomosis was technically feasible and seemed to improve mechanical strength and collagen deposition in this experimental porcine model. Further investigation is warranted. Copyright
Authors: Juliette C Slieker; Niels Komen; Niels A P Komen; Guido H Mannaerts; Tom M Karsten; Paul Willemsen; Magdalena Murawska; Johannes Jeekel; Johan F Lange Journal: Arch Surg Date: 2012-05
Authors: Jun Seok Park; Gyu-Seog Choi; Seon Hahn Kim; Hyeong Rok Kim; Nam Kyu Kim; Kang Young Lee; Sung Bum Kang; Ji Yeon Kim; Kil Yeon Lee; Byung Chun Kim; Byung Noe Bae; Gyung Mo Son; Sun Il Lee; Hyun Kang Journal: Ann Surg Date: 2013-04 Impact factor: 12.969