Literature DB >> 32833216

Ribopuromycylation in Coronavirus-Infected Cells.

Matthew J Brownsword1,2, Helena J Maier1, Nicolas Locker3.   

Abstract

Ribopuromycylation enables the visualization and quantitation of translation on a cellular level by immunofluorescence or in total using standard western blotting. This technique uses ribosome catalyzed puromycylation of nascent chains followed by immobilization on the ribosome by antibiotic chain elongation inhibitor emetine. Detection of puromycylated ribosome-bound nascent chains can then be achieved using a puromycin-specific antibody.

Entities:  

Keywords:  Emetine; Puromycin; Puromycylation; Ribopuromycylation; Translation

Mesh：

Substances：

Year:  2020        PMID： 32833216     DOI： 10.1007/978-1-0716-0900-2_17

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  13 in total

1.  SUnSET, a nonradioactive method to monitor protein synthesis.

Authors:  Enrico K Schmidt; Giovanna Clavarino; Maurizio Ceppi; Philippe Pierre
Journal:  Nat Methods       Date:  2009-03-22       Impact factor: 28.547

2.  Reverse genetics system for the avian coronavirus infectious bronchitis virus.

Authors:  R Casais; V Thiel; S G Siddell; D Cavanagh; P Britton
Journal:  J Virol       Date:  2001-12       Impact factor: 5.103

3.  Fiji: an open-source platform for biological-image analysis.

Authors:  Johannes Schindelin; Ignacio Arganda-Carreras; Erwin Frise; Verena Kaynig; Mark Longair; Tobias Pietzsch; Stephan Preibisch; Curtis Rueden; Stephan Saalfeld; Benjamin Schmid; Jean-Yves Tinevez; Daniel James White; Volker Hartenstein; Kevin Eliceiri; Pavel Tomancak; Albert Cardona
Journal:  Nat Methods       Date:  2012-06-28       Impact factor: 28.547

Review 4.  Inhibitors of ribosome functions.

Authors:  S Pestka
Journal:  Annu Rev Microbiol       Date:  1971       Impact factor: 15.500

5.  Emetine optimally facilitates nascent chain puromycylation and potentiates the ribopuromycylation method (RPM) applied to inert cells.

Authors:  Alexandre David; Jack R Bennink; Jonathan W Yewdell
Journal:  Histochem Cell Biol       Date:  2012-12-11       Impact factor: 4.304

6.  Nuclear translation visualized by ribosome-bound nascent chain puromycylation.

Authors:  Alexandre David; Brian P Dolan; Heather D Hickman; Jonathan J Knowlton; Giovanna Clavarino; Philippe Pierre; Jack R Bennink; Jonathan W Yewdell
Journal:  J Cell Biol       Date:  2012-04-02       Impact factor: 10.539

7.  Separate domains of G3BP promote efficient clustering of alphavirus replication complexes and recruitment of the translation initiation machinery.

Authors:  Benjamin Götte; Marc D Panas; Kirsi Hellström; Lifeng Liu; Baila Samreen; Ola Larsson; Tero Ahola; Gerald M McInerney
Journal:  PLoS Pathog       Date:  2019-06-14       Impact factor: 6.823

8.  The RiboPuromycylation Method (RPM): an Immunofluorescence Technique to Map Translation Sites at the Sub-cellular Level.

Authors:  Amandine Bastide; Jonathan W Yewdell; Alexandre David
Journal:  Bio Protoc       Date:  2018-01-05

9.  Virus-mediated compartmentalization of the host translational machinery.

Authors:  Emily A Desmet; Lynne J Anguish; John S L Parker
Journal:  mBio       Date:  2014-09-16       Impact factor: 7.867

10.  Metformin blocks MYC protein synthesis in colorectal cancer via mTOR-4EBP-eIF4E and MNK1-eIF4G-eIF4E signaling.

Authors:  Peng Shen; Lucas C Reineke; Erik Knutsen; Meng Chen; Martin Pichler; Hui Ling; George A Calin
Journal:  Mol Oncol       Date:  2018-10-15       Impact factor: 6.603
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