Literature DB >> 19305406

SUnSET, a nonradioactive method to monitor protein synthesis.

Enrico K Schmidt1, Giovanna Clavarino, Maurizio Ceppi, Philippe Pierre.   

Abstract

We developed a nonradioactive fluorescence-activated cell sorting-based assay, called surface sensing of translation (SUnSET), which allows the monitoring and quantification of global protein synthesis in individual mammalian cells and in heterogeneous cell populations. We demonstrate here, using mouse dendritic and T cells as a model, that SUnSET offers a technical alternative to classical radioactive labeling methods for the study of mRNA translation and cellular activation.

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Year:  2009        PMID： 19305406     DOI： 10.1038/nmeth.1314

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  13 in total

1.  INHIBITION OF PROTEIN SYNTHESIS BY PUROMYCIN.

Authors:  D NATHANS
Journal:  Fed Proc       Date:  1964 Sep-Oct

2.  A general approach to detect protein expression in vivo using fluorescent puromycin conjugates.

Authors:  Shelley R Starck; Harry M Green; José Alberola-Ila; Richard W Roberts
Journal:  Chem Biol       Date:  2004-07

Review 3.  Protein oligomerization in the endoplasmic reticulum.

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Journal:  Annu Rev Cell Biol       Date:  1989

4.  Dopaminergic stimulation of local protein synthesis enhances surface expression of GluR1 and synaptic transmission in hippocampal neurons.

Authors:  W Bryan Smith; Shelley R Starck; Richard W Roberts; Erin M Schuman
Journal:  Neuron       Date:  2005-03-03       Impact factor: 17.173

Review 5.  New modes of translational control in development, behavior, and disease.

Authors:  Nahum Sonenberg; Alan G Hinnebusch
Journal:  Mol Cell       Date:  2007-12-14       Impact factor: 17.970

6.  Rapamycin differentially inhibits S6Ks and 4E-BP1 to mediate cell-type-specific repression of mRNA translation.

Authors:  Andrew Y Choo; Sang-Oh Yoon; Sang Gyun Kim; Philippe P Roux; John Blenis
Journal:  Proc Natl Acad Sci U S A       Date:  2008-10-27       Impact factor: 11.205

Review 7.  mTOR and cancer: insights into a complex relationship.

Authors:  David M Sabatini
Journal:  Nat Rev Cancer       Date:  2006-08-17       Impact factor: 60.716

Review 8.  RNA granules.

Authors:  Paul Anderson; Nancy Kedersha
Journal:  J Cell Biol       Date:  2006-03-06       Impact factor: 10.539

9.  Dendritic cell aggresome-like induced structures are dedicated areas for ubiquitination and storage of newly synthesized defective proteins.

Authors:  Hugues Lelouard; Vincent Ferrand; Didier Marguet; Jacek Bania; Voahirana Camosseto; Alexandre David; Evelina Gatti; Philippe Pierre
Journal:  J Cell Biol       Date:  2004-02-23       Impact factor: 10.539

10.  Regulation of translation is required for dendritic cell function and survival during activation.

Authors:  Hugues Lelouard; Enrico K Schmidt; Voahirana Camosseto; Giovanna Clavarino; Maurizio Ceppi; Hsiang-Ting Hsu; Philippe Pierre
Journal:  J Cell Biol       Date:  2007-12-31       Impact factor: 10.539
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  635 in total

1.  Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin.

Authors:  Jing Liu; Yangqing Xu; Dan Stoleru; Adrian Salic
Journal:  Proc Natl Acad Sci U S A       Date:  2011-12-12       Impact factor: 11.205

2.  The molecular basis for selective inhibition of unconventional mRNA splicing by an IRE1-binding small molecule.

Authors:  Benedict C S Cross; Peter J Bond; Pawel G Sadowski; Babal Kant Jha; Jaroslav Zak; Jonathan M Goodman; Robert H Silverman; Thomas A Neubert; Ian R Baxendale; David Ron; Heather P Harding
Journal:  Proc Natl Acad Sci U S A       Date:  2012-02-06       Impact factor: 11.205

3.  Imaging of protein synthesis with puromycin.

Authors:  Craig A Goodman; Philippe Pierre; Troy A Hornberger
Journal:  Proc Natl Acad Sci U S A       Date:  2012-03-23       Impact factor: 11.205

4.  Transfer RNA's latest port of call.

Authors:  Manuel A S Santos; Omar Orellana; Michael Ibba
Journal:  RNA Biol       Date:  2010-09-01       Impact factor: 4.652

5.  Novel insights into the regulation of skeletal muscle protein synthesis as revealed by a new nonradioactive in vivo technique.

Authors:  Craig A Goodman; Danielle M Mabrey; John W Frey; Man Hing Miu; Enrico K Schmidt; Philippe Pierre; Troy A Hornberger
Journal:  FASEB J       Date:  2010-12-08       Impact factor: 5.191

6.  Insulin-Like Growth Factor II Targets the mTOR Pathway to Reverse Autism-Like Phenotypes in Mice.

Authors:  Adam B Steinmetz; Sarah A Stern; Amy S Kohtz; Giannina Descalzi; Cristina M Alberini
Journal:  J Neurosci       Date:  2017-12-07       Impact factor: 6.167

7.  mTOR complex 2 phosphorylates IMP1 cotranslationally to promote IGF2 production and the proliferation of mouse embryonic fibroblasts.

Authors:  Ning Dai; Jan Christiansen; Finn C Nielsen; Joseph Avruch
Journal:  Genes Dev       Date:  2013-02-01       Impact factor: 11.361

8.  TAR DNA-Binding Protein 43 and Disrupted in Schizophrenia 1 Coaggregation Disrupts Dendritic Local Translation and Mental Function in Frontotemporal Lobar Degeneration.

Authors:  Ryo Endo; Noriko Takashima; Yoko Nekooki-Machida; Yusuke Komi; Kelvin Kai-Wan Hui; Masaki Takao; Hiroyasu Akatsu; Shigeo Murayama; Akira Sawa; Motomasa Tanaka
Journal:  Biol Psychiatry       Date:  2018-03-29       Impact factor: 13.382

Review 9.  The Growing Toolbox for Protein Synthesis Studies.

Authors:  Shintaro Iwasaki; Nicholas T Ingolia
Journal:  Trends Biochem Sci       Date:  2017-05-28       Impact factor: 13.807

10.  Ribosomal protein S6 kinase 1 signaling in prefrontal cortex controls depressive behavior.

Authors:  Jason M Dwyer; Jaime G Maldonado-Avilés; Ashley E Lepack; Ralph J DiLeone; Ronald S Duman
Journal:  Proc Natl Acad Sci U S A       Date:  2015-04-27       Impact factor: 11.205
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