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Literature DB >> 3278300

Mutational analysis of the tobacco mosaic virus 5'-leader for altered ability to enhance translation.

D R Gallie¹, D E Sleat, J W Watts, P C Turner, T M Wilson.

Abstract

Mutational analysis of the 5'-untranslated leader sequence (omega) of tobacco mosaic virus (TMV) was carried out to determine those sequences necessary for the translational enhancement associated with omega. Five deletion mutants, a single base substitution, and a 25 base replacement mutant were tested for alterations in omega's ability to enhance expression of beta-glucuronidase (GUS) mRNA in tobacco mesophyll protoplasts and Escherichia coli or chloramphenicol acetyltransferase (CAT) mRNA in Xenopus laevis oocytes. Alteration of an eight base subsequence required for the binding of a second ribosome resulted in the loss of translational enhancement in X. laevis oocytes but not in protoplasts. Substantial increases in enhancement were observed for several of the mutants in E. coli.

Entities: Gene Species

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Year: 1988 PMID： 3278300 PMCID： PMC334725 DOI： 10.1093/nar/16.3.883

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

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