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Literature DB >> 6287164

Genetic map of the crown gall suppressive IncW plasmid pSa.

Abstract

A genetic map of the W incompatibility group plasmid pSa has been prepared through the construction of deletion derivatives of pSa and the cloning of various fragments of pSa in pBR322. Phenotypic analysis of these derivatives has identified the location of genes encoding resistance to chloramphenicol, sulfonamides, spectinomycin, streptomycin, kanamycin, gentamycin, and tobramycin. Information sufficient for the replication of the plasmid in both Escherichia coli and Agrobacterium tumefaciens is contained within a 4 kilobase pair region. Two regions have been identified as involved in the transfer of the plasmid; one of these regions is also involved in the inhibition of oncogenesis by pSa when it is present in an oncogenic strain of A. tumefaciens. Certain of the deletion derivatives of pSa are potential vectors for the cloning and analysis of A. tumefaciens Ti plasmid DNA.

Entities: Chemical Species

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Year: 1982 PMID： 6287164 DOI： 10.1007/bf00422905

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

23 in total

1. Studies on Agrobacterium tumefaciens. VIII. Avirulence induced by temperature and ethidium bromide.

Authors: B C Lin; C I Kado
Journal: Can J Microbiol Date: 1977-11 Impact factor: 2.419

2. Structure of deletion derivatives of a recombinant plasmid containing the transposable element Tn9 in the spacer sequence of Xenopus laevis 5S DNA.

Authors: N V Fedoroff
Journal: Cold Spring Harb Symp Quant Biol Date: 1979

Genetic map of the crown gall suppressive IncW plasmid pSa.

1. Studies on Agrobacterium tumefaciens. VIII. Avirulence induced by temperature and ethidium bromide.

2. Structure of deletion derivatives of a recombinant plasmid containing the transposable element Tn9 in the spacer sequence of Xenopus laevis 5S DNA.

3. Molecular cloning as a tool to the analysis of the Ti plasmids of Agrobacterium tumefaciens [proceedings].

4. Compatibility groups among fi - R factors.

5. Deletion mutants of Xenopus laevis 5S ribosomal DNA.

6. Host range conferred by the virulence-specifying plasmid of Agrobacterium tumefaciens.

7. Basic characterization of W-pili.

8. Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.

9. Plasmid-dependent attachment of Agrobacterium tumefaciens to plant tissue culture cells.

10. Diversity among B6 strains of Agrobacterium tumefaciens.

1. A comparison of eukaryotic viral 5'-leader sequences as enhancers of mRNA expression in vivo.

2. Convenience of plasmid R6K higher-copy-number deletion derivative for cloning of larger DNA fragments.

3. Long-term starvation-induced loss of antibiotic resistance in bacteria.

4. Identification of Phytophthora citrophthora with Cloned DNA Probes.

5. N conjugative transfer system of plasmid pCU1.

6. Activity of the Agrobacterium T-DNA transfer machinery is affected by virB gene products.

7. A functional map of the nopaline catabolism genes on the Ti plasmid of Agrobacterium tumefaciens C58.

8. Analysis of Agrobacterium tumefaciens plasmid pTiC58 replication region with a novel high-copy-number derivative.

9. Characterization of the replication and stability regions of Agrobacterium tumefaciens plasmid pTAR.

10. Inhibition of Agrobacterium tumefaciens oncogenicity by the osa gene of pSa.