Literature DB >> 3275610

Efficient expression of the yeast metallothionein gene in Escherichia coli.

T Berka1, A Shatzman, J Zimmerman, J Strickler, M Rosenberg.   

Abstract

The yeast metallothionein gene CUP1 was cloned into a bacterial expression system to achieve efficient, controlled expression of the stable, unprocessed protein product. The Escherichia coli-synthesized yeast metallothionein bound copper, cadmium, and zinc, indicating that the protein was functional. Furthermore, E. coli cells expressing CUP1 acquired a new, inducible ability to selectively sequester heavy metal ions from the growth medium.

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Year:  1988        PMID： 3275610      PMCID： PMC210600          DOI： 10.1128/jb.170.1.21-26.1988

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  28 in total

1.  Gene control of coppersensitivity in Saccharomyces.

Authors:  A BRENES-POMALES; G LINDEGREN; C C LINDEGREN
Journal:  Nature       Date:  1955-10-29       Impact factor: 49.962

2.  Chromosome Mapping in Saccharomyces: Centromere-Linked Genes.

Authors:  D C Hawthorne; R K Mortimer
Journal:  Genetics       Date:  1960-08       Impact factor: 4.562

3.  [The biosynthesis of beta-galactosidase (lactase) in Escherichia coli; the specificity of induction].

Authors:  J MONOD; G COHEN-BAZIRE; M COHN
Journal:  Biochim Biophys Acta       Date:  1951-11

4.  Characterization of Cd, Zn-thionein (metallothionein) isolated from rat and chicken liver.

Authors:  U Weser; H Rupp; F Donay; F Linnemann; W Voelter; W Voetsch; G Jung
Journal:  Eur J Biochem       Date:  1973-11-01

5.  A cadmium-resistant variant of the Chinese hamster (CHO) cell with increased metallothionein induction capacity.

Authors:  C E Hildebrand; R A Tobey; E W Campbell; M D Enger
Journal:  Exp Cell Res       Date:  1979-12       Impact factor: 3.905

6.  A naturally occurring Cu-thionein in Saccharomyces cerevisiae.

Authors:  R Prinz; U Weser
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1975-06

7.  Tandem gene amplification mediates copper resistance in yeast.

Authors:  S Fogel; J W Welch
Journal:  Proc Natl Acad Sci U S A       Date:  1982-09       Impact factor: 11.205

8.  Cloning and sequence analysis of two monkey metallothionein cDNAs.

Authors:  C J Schmidt; D H Hamer
Journal:  Gene       Date:  1983-09       Impact factor: 3.688

9.  The use of pKc30 and its derivatives for controlled expression of genes.

Authors:  M Rosenberg; Y S Ho; A Shatzman
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

10.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
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  10 in total

1.  Development of bacterium-based heavy metal biosorbents: enhanced uptake of cadmium and mercury by Escherichia coli expressing a metal binding motif.

Authors:  M Pazirandeh; B M Wells; R L Ryan
Journal:  Appl Environ Microbiol       Date:  1998-10       Impact factor: 4.792

2.  Construction and characterization of Escherichia coli genetically engineered for bioremediation of Hg(2+)-contaminated environments.

Authors:  S Chen; D B Wilson
Journal:  Appl Environ Microbiol       Date:  1997-06       Impact factor: 4.792

3.  Metalloadsorption by Escherichia coli cells displaying yeast and mammalian metallothioneins anchored to the outer membrane protein LamB.

Authors:  C Sousa; P Kotrba; T Ruml; A Cebolla; V De Lorenzo
Journal:  J Bacteriol       Date:  1998-05       Impact factor: 3.490

4.  Cytoplasmic expression of a soluble synthetic mammalian metallothionein-alpha domain in Escherichia coli. Enhanced tolerance and accumulation of cadmium.

Authors:  Y Li; W Cockburn; J Kilpatrick; G C Whitelam
Journal:  Mol Biotechnol       Date:  2000-11       Impact factor: 2.695

5.  Ubiquitin fusion augments the yield of cloned gene products in Escherichia coli.

Authors:  T R Butt; S Jonnalagadda; B P Monia; E J Sternberg; J A Marsh; J M Stadel; D J Ecker; S T Crooke
Journal:  Proc Natl Acad Sci U S A       Date:  1989-04       Impact factor: 11.205

6.  Expression of a Neurospora crassa metallothionein and its variants in Escherichia coli.

Authors:  F M Romeyer; F A Jacobs; R Brousseau
Journal:  Appl Environ Microbiol       Date:  1990-09       Impact factor: 4.792

7.  Roles of the conserved serines of metallothionein in cadmium binding.

Authors:  T Emoto; M Kurasaki; S Oikawa; M Suzuki-Kurasaki; M Okabe; F Yamasaki; Y Kojima
Journal:  Biochem Genet       Date:  1996-06       Impact factor: 1.890

8.  Expression of the Neurospora crassa metallothionein gene in Escherichia coli and its effect on heavy-metal uptake.

Authors:  M Pazirandeh; L A Chrisey; J M Mauro; J R Campbell; B P Gaber
Journal:  Appl Microbiol Biotechnol       Date:  1995-11       Impact factor: 4.813

9.  Characterization of mercury bioremediation by transgenic bacteria expressing metallothionein and polyphosphate kinase.

Authors:  Oscar N Ruiz; Derry Alvarez; Gloriene Gonzalez-Ruiz; Cesar Torres
Journal:  BMC Biotechnol       Date:  2011-08-12       Impact factor: 2.563

10.  Cyanobacterial promoted enrichment of rare earth elements europium, samarium and neodymium and intracellular europium particle formation.

Authors:  Christian B Fischer; Susanne Körsten; Liz M Rösken; Felix Cappel; Christian Beresko; Georg Ankerhold; Andreas Schönleber; Stefan Geimer; Dennis Ecker; Stefan Wehner
Journal:  RSC Adv       Date:  2019-10-11       Impact factor: 4.036
  10 in total

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