Literature DB >> 9172366

Construction and characterization of Escherichia coli genetically engineered for bioremediation of Hg(2+)-contaminated environments.

S Chen1, D B Wilson.   

Abstract

Escherichia coli strains were genetically engineered to express an Hg2+ transport system and metallothionein. Overexpression of a glutathione S-transferase fusion protein of Saccharomyces cerevisiae or pea metallothionein significantly increased the bioaccumulation of Hg2+ transported by MerT and MerP and protected the cells from the accumulated Hg2+. The recombinant strains have excellent properties for bioremediation of Hg(2+)-contaminated environments.

Entities:  

Mesh：

Substances：

Year:  1997        PMID： 9172366      PMCID： PMC168538          DOI： 10.1128/aem.63.6.2442-2445.1997

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  12 in total

1.  Extraction of mercury from groundwater using immobilized algae.

Authors:  N P Barkley
Journal:  J Air Waste Manage Assoc       Date:  1991-10

Review 2.  Overview of metallothionein.

Authors:  J H Kägi
Journal:  Methods Enzymol       Date:  1991       Impact factor: 1.600

3.  High efficiency transformation of E. coli by high voltage electroporation.

Authors:  W J Dower; J F Miller; C W Ragsdale
Journal:  Nucleic Acids Res       Date:  1988-07-11       Impact factor: 16.971

4.  Culture medium for enterobacteria.

Authors:  F C Neidhardt; P L Bloch; D F Smith
Journal:  J Bacteriol       Date:  1974-09       Impact factor: 3.490

5.  Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.

Authors:  D B Smith; K S Johnson
Journal:  Gene       Date:  1988-07-15       Impact factor: 3.688

6.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors:  C Yanisch-Perron; J Vieira; J Messing
Journal:  Gene       Date:  1985       Impact factor: 3.688

7.  The role of cysteine residues in the transport of mercuric ions by the Tn501 MerT and MerP mercury-resistance proteins.

Authors:  A P Morby; J L Hobman; N L Brown
Journal:  Mol Microbiol       Date:  1995-07       Impact factor: 3.501

8.  Expression of the pea gene PSMTA in E. coli. Metal-binding properties of the expressed protein.

Authors:  A M Tommey; J Shi; W P Lindsay; P E Urwin; N J Robinson
Journal:  FEBS Lett       Date:  1991-11-04       Impact factor: 4.124

9.  Efficient expression of the yeast metallothionein gene in Escherichia coli.

Authors:  T Berka; A Shatzman; J Zimmerman; J Strickler; M Rosenberg
Journal:  J Bacteriol       Date:  1988-01       Impact factor: 3.490

10.  Purification and functional characterization of MerD. A coregulator of the mercury resistance operon in gram-negative bacteria.

Authors:  D Mukhopadhyay; H R Yu; G Nucifora; T K Misra
Journal:  J Biol Chem       Date:  1991-10-05       Impact factor: 5.157
View more
  17 in total

1.  Construction and characterization of an Escherichia coli strain genetically engineered for Ni(II) bioaccumulation.

Authors:  R Krishnaswamy; D B Wilson
Journal:  Appl Environ Microbiol       Date:  2000-12       Impact factor: 4.792

2.  Genetic engineering of Escherichia coli for enhanced uptake and bioaccumulation of mercury.

Authors:  W Bae; R K Mehra; A Mulchandani; W Chen
Journal:  Appl Environ Microbiol       Date:  2001-11       Impact factor: 4.792

3.  Generation of mercury-hyperaccumulating plants through transgenic expression of the bacterial mercury membrane transport protein MerC.

Authors:  Yoshito Sasaki; Takahiko Hayakawa; Chihiro Inoue; Atsushi Miyazaki; Simon Silver; Tomonobu Kusano
Journal:  Transgenic Res       Date:  2006-07-09       Impact factor: 2.788

4.  Development of bacterium-based heavy metal biosorbents: enhanced uptake of cadmium and mercury by Escherichia coli expressing a metal binding motif.

Authors:  M Pazirandeh; B M Wells; R L Ryan
Journal:  Appl Environ Microbiol       Date:  1998-10       Impact factor: 4.792

5.  Cadmium accumulation and DNA homology with metal resistance genes in sulfate-reducing bacteria.

Authors:  Naghma Naz; Hilary K Young; Nuzhat Ahmed; Geoffrey M Gadd
Journal:  Appl Environ Microbiol       Date:  2005-08       Impact factor: 4.792

6.  Characterization of cadmium uptake in Lactobacillus plantarum and isolation of cadmium and manganese uptake mutants.

Authors:  Z Hao; H R Reiske; D B Wilson
Journal:  Appl Environ Microbiol       Date:  1999-11       Impact factor: 4.792

7.  Metalloadsorption by Escherichia coli cells displaying yeast and mammalian metallothioneins anchored to the outer membrane protein LamB.

Authors:  C Sousa; P Kotrba; T Ruml; A Cebolla; V De Lorenzo
Journal:  J Bacteriol       Date:  1998-05       Impact factor: 3.490

8.  Removal of mercury from chloralkali electrolysis wastewater by a mercury-resistant Pseudomonas putida strain.

Authors:  H von Canstein; Y Li; K N Timmis; W D Deckwer; I Wagner-Döbler
Journal:  Appl Environ Microbiol       Date:  1999-12       Impact factor: 4.792

9.  Bioaccumulation of copper ions by Escherichia coli expressing vanabin genes from the vanadium-rich ascidian Ascidia sydneiensis samea.

Authors:  Tatsuya Ueki; Yasuhisa Sakamoto; Nobuo Yamaguchi; Hitoshi Michibata
Journal:  Appl Environ Microbiol       Date:  2003-11       Impact factor: 4.792

10.  Cloning and overexpression of alkaline phosphatase PhoK from Sphingomonas sp. strain BSAR-1 for bioprecipitation of uranium from alkaline solutions.

Authors:  Kayzad S Nilgiriwala; Anuradha Alahari; Amara Sambasiva Rao; Shree Kumar Apte
Journal:  Appl Environ Microbiol       Date:  2008-07-18       Impact factor: 4.792
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.