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Literature DB >> 1060178

Molecular vehicle properties of the broad host range plasmid RK2.

Abstract

The pladmid RK2 is stably maintained in a broad range of gram-negative bacteria. The RK2 DNA has a single Eco RI restriction site. The insertion of a DNA fragment into this site does not interfere with either plasmid maintenance or self-transmissibility. Because RK2 has a broad host range, it should be useful for the construction in vitro of hybrid plasmid molecules capable of being established by conjugal transfer or transformation into many genera of gram-negative organisms.

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Year: 1975 PMID： 1060178 DOI： 10.1126/science.1060178

Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728

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Cited

11 in total

1. Transposition of DNA inserted into deletions of the Tn5 kanamycin resistance element.

Authors: R Meyer; G Boch; J Shapiro
Journal: Mol Gen Genet Date: 1979-03-09

2. Measuring genetic stability in bacteria of potential use in genetic engineering.

Authors: M V Walter; A Porteous; R J Seidler
Journal: Appl Environ Microbiol Date: 1987-01 Impact factor: 4.792

3. Isolation and characterization of kikA, a region on IncN group plasmids that determines killing of Klebsiella oxytoca.

Authors: P N Hengen; D Denicourt; V N Iyer
Journal: J Bacteriol Date: 1992-05 Impact factor: 3.490

Molecular vehicle properties of the broad host range plasmid RK2.

1. Transposition of DNA inserted into deletions of the Tn5 kanamycin resistance element.

2. Measuring genetic stability in bacteria of potential use in genetic engineering.

3. Isolation and characterization of kikA, a region on IncN group plasmids that determines killing of Klebsiella oxytoca.

4. Physical and genetic studies with restriction endonucleases on the broad host-range plasmid RK2.

5. Gene transfer system for Rhodopseudomonas viridis.

6. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.

7. Genetic organization of the broad-host-range IncP-1 plasmid R751.

8. Broad-host-range IncP-4 plasmid R1162: effects of deletions and insertions on plasmid maintenance and host range.

9. Expression of incompatibility by derivatives of the broad host-range inc P-1 plasmid RK2.

10. Multiple mechanisms for expression of incompatibility by broad-host-range plasmid RK2.