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Literature DB >> 327261

Detection of messenger RNA from the isoleucine--valine operons of Salmonella typhimurium by heterologous DNA-RNA hybridization: involvement of transfer RNA in transcriptional repression.

Abstract

A hybridization assay using Escherichia coli K-12 DNA isolated from the specialized transducing bacteriophage gammaCI857St68h80 dilv was used to examine the rate of synthesis of the messenger RNA's (mRNA) derived from the isoleucine-valine (ilv) gene cluster of Salmonella typhimurium. In all cases examined, changes in ilv enzyme levels could be correlated with changes in the rate of synthesis of ilv mRNA. Several well characterized regulatory mutants of S. typhimurium had rates of synthesis of ilv mRNA 3 to 8-fold higher than the repressed wild-type strain. The increased rates of ilv mRNA synthesis found in a hisT strain as well as in isoleucyl-and leucyl-tRNA SYNTHETASE MUTANTS, STRONGLY SUGGESTS A ROLE FOR BRANCHED-CHAIN AMINOACYL-TRNA's in transcriptional control.

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Year: 1977 PMID： 327261 DOI： 10.1007/bf00338686

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

26 in total

8. Derepressed levels of the isoleucine-valine and leucine enzymes in his T 1504, a strain of Salmonella typhimurium with altered leucine transfer ribonucleic acid.

Authors: A A Rizzino; R S Bresalier; M Freundlich
Journal: J Bacteriol Date: 1974-02 Impact factor: 3.490

9. REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. 3. MAP ORDER OF THE STRUCTURAL GENES AND OPERATOR GENES.

Authors: T RAMAKRISHNAN; E A ADELBERG
Journal: J Bacteriol Date: 1965-03 Impact factor: 3.490

10. Isolation and characterization of specialized phi80 transducing phages carrying regions of the Salmonella typhimurium trp operon.

Authors: R M Denney; C Yanofsky
Journal: J Bacteriol Date: 1974-05 Impact factor: 3.490

3 in total

Detection of messenger RNA from the isoleucine--valine operons of Salmonella typhimurium by heterologous DNA-RNA hybridization: involvement of transfer RNA in transcriptional repression.

1. Mutants of Escherichia coli requiring methionine or vitamin B12.

2. The regulation of the ilv ADGE operon: evidence for positive control by threonine deaminase.

3. Isoleucine and valine metabolism in Escherichia coli. XX. Multiple forms of acetohydroxy acid synthetase.

4. Structural genes for a newly recognized acetolactate synthase in Escherichia coli K-12.

5. Specific binding of leucyl transfer RNA to an immature form of L-threonine deaminase: its implications in repression.

6. Transcriptional control of the isoleucine-valine messenger RNA's in E. coli K-12.

7. Kinetic complexity of RNA molecules.

8. Derepressed levels of the isoleucine-valine and leucine enzymes in his T 1504, a strain of Salmonella typhimurium with altered leucine transfer ribonucleic acid.

9. REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. 3. MAP ORDER OF THE STRUCTURAL GENES AND OPERATOR GENES.

10. Isolation and characterization of specialized phi80 transducing phages carrying regions of the Salmonella typhimurium trp operon.

1. A site of action for tRNA mediated regulation of the ilvOEDA operon of Escherichia coli K12.

Review 2. Linkage map of Salmonella typhimurium, edition V.

3. Genetically determined differences in concentrations of isoaccepting tRNAs in Escherichia coli.