Literature DB >> 1102933

Transcriptional control of the isoleucine-valine messenger RNA's in E. coli K-12.

G J Childs, M Freundlich.   

Abstract

Hybridization of messenger ribonucleic acid (mRNA) isolated from Escherichia Coli K-12 to deoxyribonucleic acid (DNA) from lambdaCI857st68h80dilv was used to detect isoleucine-valine (ilv) specific mRNA. A number of strains partially constitutive for the isoleucine-valine enzymes had levels of ilv mRNA 2 to 3-fold higher than the parent strain. Starvation for any of the branched-chain amino acids resulted in a 20 to 23-fold increase in ilv mRNA as compared to repressed levels. These differences were not due to altered growth rates or to changes in the stability of ilv mRNA. These data indicate that regulation of the isoleucine-valine enzymes by multivalent repression occurs mainly at the level of transcription. Kinetics of elongation of ilv mRNA after repression are consistent with the assumption that the mechanism of multivalent repression involves the prevention of further initiations by RNA polymerase.

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Year:  1975        PMID： 1102933     DOI： 10.1007/bf00269352

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  30 in total

1.  Mutants of Escherichia coli requiring methionine or vitamin B12.

Authors:  B D DAVIS; E S MINGIOLI
Journal:  J Bacteriol       Date:  1950-07       Impact factor: 3.490

2.  Regulation of isoleucine-valine biosynthesis in an ilvDAC deletion strain of Escherichia coli K-12.

Authors:  E L Kline; C S Brown; W G Coleman; H E Umbarger
Journal:  Biochem Biophys Res Commun       Date:  1974-04-23       Impact factor: 3.575

3.  Regulation of in vitro transcription of the tryptophan operon by purified RNA polymerase in the presence of partially purified repressor and tryptophan.

Authors:  J K Rose; C L Squires; C Yanofsky; H L Yang; G Zubay
Journal:  Nat New Biol       Date:  1973-10-03

4.  Ordering of mutant sites in the isoleucine-valine genes of Escherichia coli by use of merogenotes derived from F 14 : a new procedure for fine-structure mapping.

Authors:  N J Marsh; D E Duggan
Journal:  J Bacteriol       Date:  1972-02       Impact factor: 3.490

5.  Isoleucine and valine metabolism in Escherichia coli. XX. Multiple forms of acetohydroxy acid synthetase.

Authors:  J M Blatt; W J Pledger; H E Umbarger
Journal:  Biochem Biophys Res Commun       Date:  1972-07-25       Impact factor: 3.575

6.  Specific binding of leucyl transfer RNA to an immature form of L-threonine deaminase: its implications in repression.

Authors:  G W Hatfield; R O Burns
Journal:  Proc Natl Acad Sci U S A       Date:  1970-08       Impact factor: 11.205

7.  The periodicity of RNA polymerase initiations: a new regulatory feature of transcription.

Authors:  R F Baker; C Yanofsky
Journal:  Proc Natl Acad Sci U S A       Date:  1968-05       Impact factor: 11.205

8.  Isoleucine and valine metabolism of Escherichia coli. XVI. Pattern of multivalent repression in strain K-12.

Authors:  S B Dwyer; H E Umbarger
Journal:  J Bacteriol       Date:  1968-05       Impact factor: 3.490

9.  Derepressed levels of the isoleucine-valine and leucine enzymes in his T 1504, a strain of Salmonella typhimurium with altered leucine transfer ribonucleic acid.

Authors:  A A Rizzino; R S Bresalier; M Freundlich
Journal:  J Bacteriol       Date:  1974-02       Impact factor: 3.490

10.  REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. 3. MAP ORDER OF THE STRUCTURAL GENES AND OPERATOR GENES.

Authors:  T RAMAKRISHNAN; E A ADELBERG
Journal:  J Bacteriol       Date:  1965-03       Impact factor: 3.490
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  5 in total

Review 1.  Growth inhibition as a consequence of antagonism between related amino acids: effect of valine in Escherichia coli K-12.

Authors:  M De Felice; M Levinthal; M Iaccarino; J Guardiola
Journal:  Microbiol Rev       Date:  1979-03

2.  Dual autogenous regulatory role of threonine deaminase in Escherichia coli K-12.

Authors:  J Guardiola; F Cervone; A Lamberti; M Levinthal; M Laccarino
Journal:  Mol Gen Genet       Date:  1978-02-07

3.  Cloning and expression of the ilvB gene of Escherichia coli K-12.

Authors:  T Newman; P Friden; A Sutton; M Freundlich
Journal:  Mol Gen Genet       Date:  1982

4.  Mutations in genes cpxA and cpxB of Escherichia coli K-12 cause a defect in acetohydroxyacid synthase I function in vivo.

Authors:  A Sutton; T Newman; J McEwen; P M Silverman; M Freundlich
Journal:  J Bacteriol       Date:  1982-08       Impact factor: 3.490

5.  Detection of messenger RNA from the isoleucine--valine operons of Salmonella typhimurium by heterologous DNA-RNA hybridization: involvement of transfer RNA in transcriptional repression.

Authors:  G Childs; F Sonnenberg; M Freundlich
Journal:  Mol Gen Genet       Date:  1977-03-07
  5 in total

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