Literature DB >> 32665966

Implementing VIPER for Imaging Cellular Proteins by Fluorescence Microscopy.

Julia K Doh1, Caroline A Enns2, Kimberly E Beatty1,3.   

Abstract

Genetically-encoded tags are useful tools for multicolor and multi-scale cellular imaging. Versatile Interacting Peptide (VIP) tags, such as VIPER, are new genetically-encoded tags that can be used in various imaging applications. VIP tags consist of a coiled-coil heterodimer, with one peptide serving as the genetic tag and the other ("probe peptide") delivering a reporter compatible with imaging. Heterodimer formation is rapid and specific, allowing proteins to be selectively labeled for live-cell and fixed-cell imaging. In this Bio-Protocol, we include a detailed guide for implementing the VIPER technology for imaging receptors on live cells and intracellular targets in fixed cells. This protocol is complemented by two other Bio-Protocols outlining the use of VIPER (Doh et al., 2019a and 2019b).

Entities:  

Keywords:  Biochemistry; Cell biology; Fluorescence; Labeling; Microscopy; Peptide; Protein tag

Year:  2019        PMID： 32665966      PMCID： PMC7360171          DOI： 10.21769/bioprotoc.3413

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  43 in total

1.  An analysis of protein domain linkers: their classification and role in protein folding.

Authors:  Richard A George; Jaap Heringa
Journal:  Protein Eng       Date:  2002-11

2.  Rapid Covalent Fluorescence Labeling of Membrane Proteins on Live Cells via Coiled-Coil Templated Acyl Transfer.

Authors:  Ulrike Reinhardt; Jonathan Lotze; Karin Mörl; Annette G Beck-Sickinger; Oliver Seitz
Journal:  Bioconjug Chem       Date:  2015-09-24       Impact factor: 4.774

Review 3.  The fluorescent toolbox for assessing protein location and function.

Authors:  Ben N G Giepmans; Stephen R Adams; Mark H Ellisman; Roger Y Tsien
Journal:  Science       Date:  2006-04-14       Impact factor: 47.728

4.  An engineered protein tag for multiprotein labeling in living cells.

Authors:  Arnaud Gautier; Alexandre Juillerat; Christian Heinis; Ivan Reis Corrêa; Maik Kindermann; Florent Beaufils; Kai Johnsson
Journal:  Chem Biol       Date:  2008-02

5.  Design and use of fluorescent fusion proteins in cell biology.

Authors:  Erik Snapp
Journal:  Curr Protoc Cell Biol       Date:  2005-07

6.  Coiled-coil tag--probe system for quick labeling of membrane receptors in living cell.

Authors:  Yoshiaki Yano; Akiko Yano; Shinya Oishi; Yukihiko Sugimoto; Gozoh Tsujimoto; Nobutaka Fujii; Katsumi Matsuzaki
Journal:  ACS Chem Biol       Date:  2008-06-20       Impact factor: 5.100

Review 7.  Imaging live-cell dynamics and structure at the single-molecule level.

Authors:  Zhe Liu; Luke D Lavis; Eric Betzig
Journal:  Mol Cell       Date:  2015-05-21       Impact factor: 17.970

Review 8.  Pick a Tag and Explore the Functions of Your Pet Protein.

Authors:  Giel Vandemoortele; Sven Eyckerman; Kris Gevaert
Journal:  Trends Biotechnol       Date:  2019-04-27       Impact factor: 19.536

Review 9.  Imaging proteins inside cells with fluorescent tags.

Authors:  Georgeta Crivat; Justin W Taraska
Journal:  Trends Biotechnol       Date:  2011-09-15       Impact factor: 19.536

10.  Versatile Interacting Peptide (VIP) Tags for Labeling Proteins with Bright Chemical Reporters.

Authors:  Hannah K Zane; Julia K Doh; Caroline A Enns; Kimberly E Beatty
Journal:  Chembiochem       Date:  2017-01-25       Impact factor: 3.164
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  2 in total

1.  Imaging VIPER-labeled Cellular Proteins by Correlative Light and Electron Microscopy.

Authors:  Julia K Doh; Young Hwan Chang; Caroline A Enns; Claudia S Lόpez; Kimberly E Beatty
Journal:  Bio Protoc       Date:  2019-11-05

2.  Generation of CoilR Probe Peptides for VIPER-labeling of Cellular Proteins.

Authors:  Julia K Doh; Savannah J Tobin; Kimberly E Beatty
Journal:  Bio Protoc       Date:  2019-11-05
  2 in total

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