Literature DB >> 18228466

Design and use of fluorescent fusion proteins in cell biology.

Erik Snapp1.   

Abstract

This unit describes strategies for designing functional fluorescent fusion protein constructs. Such constructs can be exploited as probes of cellular environments, protein dynamics, protein life histories, protein binding partners, and markers in living cells. The properties and uses of many currently available fluorescent proteins are discussed. In addition, alternative approaches and troubleshooting guidelines are provided.

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Year:  2005        PMID： 18228466      PMCID： PMC2875081          DOI： 10.1002/0471143030.cb2104s27

Source DB:  PubMed          Journal:  Curr Protoc Cell Biol        ISSN： 1934-2616


  46 in total

Review 1.  Lighting up cells: labelling proteins with fluorophores.

Authors:  Atsushi Miyawaki; Asako Sawano; Takako Kogure
Journal:  Nat Cell Biol       Date:  2003-09       Impact factor: 28.824

2.  A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling.

Authors:  Satoshi Karasawa; Toshio Araki; Miki Yamamoto-Hino; Atsushi Miyawaki
Journal:  J Biol Chem       Date:  2003-06-19       Impact factor: 5.157

3.  Why are both ends of the polypeptide chain on the outside of proteins?

Authors:  Sven Hovmöller; Tuping Zhou
Journal:  Proteins       Date:  2004-05-01

4.  Selective photolabeling of proteins using photoactivatable GFP.

Authors:  George H Patterson; Jennifer Lippincott-Schwartz
Journal:  Methods       Date:  2004-04       Impact factor: 3.608

5.  An improved cyan fluorescent protein variant useful for FRET.

Authors:  Mark A Rizzo; Gerald H Springer; Butch Granada; David W Piston
Journal:  Nat Biotechnol       Date:  2004-02-29       Impact factor: 54.908

Review 6.  Calmodulin.

Authors:  A S Manalan; C B Klee
Journal:  Adv Cyclic Nucleotide Protein Phosphorylation Res       Date:  1984

7.  Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.

Authors:  Mark A Sheff; Kurt S Thorn
Journal:  Yeast       Date:  2004-06       Impact factor: 3.239

8.  Absolute values of ras p21 defined by direct binding liquid competition radioimmunoassays.

Authors:  P Horan Hand; V Vilasi; A Caruso; J Schlom
Journal:  Biochim Biophys Acta       Date:  1987-02-27

9.  Density of newly synthesized plasma membrane proteins in intracellular membranes. I. Stereological studies.

Authors:  G Griffiths; G Warren; P Quinn; O Mathieu-Costello; H Hoppeler
Journal:  J Cell Biol       Date:  1984-06       Impact factor: 10.539

10.  Formation of stacked ER cisternae by low affinity protein interactions.

Authors:  Erik L Snapp; Ramanujan S Hegde; Maura Francolini; Francesca Lombardo; Sara Colombo; Emanuela Pedrazzini; Nica Borgese; Jennifer Lippincott-Schwartz
Journal:  J Cell Biol       Date:  2003-10-27       Impact factor: 10.539
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  41 in total

1.  Expansion microscopy of C. elegans.

Authors:  Chih-Chieh Jay Yu; Nicholas C Barry; Asmamaw T Wassie; Anubhav Sinha; Abhishek Bhattacharya; Shoh Asano; Chi Zhang; Fei Chen; Oliver Hobert; Miriam B Goodman; Gal Haspel; Edward S Boyden
Journal:  Elife       Date:  2020-05-01       Impact factor: 8.140

Review 2.  Going Viral with Fluorescent Proteins.

Authors:  Lindsey M Costantini; Erik L Snapp
Journal:  J Virol       Date:  2015-07-22       Impact factor: 5.103

3.  Production and cleavage of a fusion protein of porcine trypsinogen and enhanced green fluorescent protein (EGFP) in Pichia pastoris.

Authors:  Hana Raschmanová; Leona Paulová; Barbora Branská; Zdeněk Knejzlík; Karel Melzoch; Karin Kovar
Journal:  Folia Microbiol (Praha)       Date:  2018-06-05       Impact factor: 2.099

Review 4.  Role of green fluorescent proteins and their variants in development of FRET-based sensors.

Authors:  Neha Soleja; Ovais Manzoor; Imran Khan; Altaf Ahmad; Mohd Mohsin
Journal:  J Biosci       Date:  2018-09       Impact factor: 1.826

Review 5.  Insights into nuclear dynamics using live-cell imaging approaches.

Authors:  Rachel B Bigley; Alexander Y Payumo; Jeffrey M Alexander; Guo N Huang
Journal:  Wiley Interdiscip Rev Syst Biol Med       Date:  2017-01-12

6.  Guide to red fluorescent proteins and biosensors for flow cytometry.

Authors:  Kiryl D Piatkevich; Vladislav V Verkhusha
Journal:  Methods Cell Biol       Date:  2011       Impact factor: 1.441

7.  Generation of transgenic mouse fluorescent reporter lines for studying hematopoietic development.

Authors:  Andrei M Vacaru; Joseph Vitale; Johnathan Nieves; Margaret H Baron
Journal:  Methods Mol Biol       Date:  2014

8.  Novel Application of Red Fluorescent Protein (DsRed-Express) for the Study of Functional Dynamics of Nuclear Receptors.

Authors:  Amit K Dash; Ashutosh S Yende; Rakesh K Tyagi
Journal:  J Fluoresc       Date:  2017-05-03       Impact factor: 2.217

Review 9.  Enzyme-targeted fluorescent small-molecule probes for bacterial imaging.

Authors:  Andrew P Marshall; Joshua D Shirley; Erin E Carlson
Journal:  Curr Opin Chem Biol       Date:  2020-08-13       Impact factor: 8.822

10.  Imaging Subcellular Structures in the Living Zebrafish Embryo.

Authors:  Peter Engerer; Gabriela Plucinska; Rachel Thong; Laura Trovò; Dominik Paquet; Leanne Godinho
Journal:  J Vis Exp       Date:  2016-04-02       Impact factor: 1.355
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