Rabiye Altinbaş1, Ayşe Barış2, Sümeyye Şen3, Recep Öztürk4, Nuri Kiraz5. 1. Department of Microbiology, Division of Mycology, Eskişehir City Hospital, Eskişehir, Turkey 2. Department of Microbiology, Division of Mycology Şişli Hamidiye Etfal Training and Research Hospital, İstanbul, Turkey 3. Department of Microbiology Zonguldak Public Health, Zonguldak, Turkey 4. Department of Infectious Diseases and Clinical Microbiology, Medical School, İstanbul Medipol University, İstanbul, Turkey 5. Department of Microbiology, Medical School, Namık Kemal University, Tekirdağ, Turkey
Abstract
Background and aim: Infections caused by Candida species are significantly increasing today, and invasive Candida infections are generally associated with high mortality. Early diagnosis and identification of Candida spp. is important for the determination of antifungal agents that will be used for treatment. The aim of the present study was to provide a better regimen for Candida infections in the future. Materials and methods: TheSensititre YeastOne (SYO) method was compared with The Clinical Laboratory Standards Institute (CLSI) reference broth microdilution (BMD) testing method. Endpoints of minimal inhibitory concentrations (MICs) were determined for both methods. Results: By using both methods, MIC values of micafungin, caspofungin, voriconazole, and fluconazole were lower than amphotericin B. The values obtained with the SYO method were in high categorical agreement for ecinocandins and amphotericin B. The results of voriconazole and fluconazole were in low categorical agreement. The categorical agreement between the SYO and the BMD results at 24 h was 82.1% for VORI and 98.4% for AMB. Values obtained with SYO method for all antifungal agents were in high essential agreement with the data of the CLSI reference BMD method. The essential agreement between the SYO and the BMD results at 24 h was 94.0% for MFG and 99.0% for AMB. Conclusions: The SYO method was ready-to use, so it appeared to be easier and more efficient for Candida isolates. This work is licensed under a Creative Commons Attribution 4.0 International License.
Background and aim: Infections caused by Candida species are significantly increasing today, and invasive Candidainfections are generally associated with high mortality. Early diagnosis and identification of Candidaspp. is important for the determination of antifungal agents that will be used for treatment. The aim of the present study was to provide a better regimen for Candida infections in the future. Materials and methods: TheSensititre YeastOne (SYO) method was compared with The Clinical Laboratory Standards Institute (CLSI) reference broth microdilution (BMD) testing method. Endpoints of minimal inhibitory concentrations (MICs) were determined for both methods. Results: By using both methods, MIC values of micafungin, caspofungin, voriconazole, and fluconazole were lower than amphotericin B. The values obtained with the SYO method were in high categorical agreement for ecinocandins and amphotericin B. The results of voriconazole and fluconazole were in low categorical agreement. The categorical agreement between the SYO and the BMD results at 24 h was 82.1% for VORI and 98.4% for AMB. Values obtained with SYO method for all antifungal agents were in high essential agreement with the data of the CLSI reference BMD method. The essential agreement between the SYO and the BMD results at 24 h was 94.0% for MFG and 99.0% for AMB. Conclusions: The SYO method was ready-to use, so it appeared to be easier and more efficient for Candida isolates. This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors: M A Pfaller; S A Messer; R J Hollis; A Espinel-Ingroff; M A Ghannoum; H Plavan; S B Killian; C C Knapp Journal: Diagn Microbiol Infect Dis Date: 1998-08 Impact factor: 2.803
Authors: A Espinel-Ingroff; M Pfaller; S A Messer; C C Knapp; S Killian; H A Norris; M A Ghannoum Journal: J Clin Microbiol Date: 1999-03 Impact factor: 5.948
Authors: Peter G Pappas; John H Rex; Jeannette Lee; Richard J Hamill; Robert A Larsen; William Powderly; Carol A Kauffman; Newton Hyslop; Julie E Mangino; Stanley Chapman; Harold W Horowitz; John E Edwards; William E Dismukes Journal: Clin Infect Dis Date: 2003-08-14 Impact factor: 9.079