Yilun Zhu1, Yuchi Qiu2, Weitao Chen3, Qing Nie4, Arthur D Lander5. 1. Department of Developmental and Cell Biology, University of California Irvine, Irvine, CA 92697, USA; Center for Complex Biological Systems, University of California Irvine, Irvine, CA 92697, USA. 2. Department of Mathematics, University of California Irvine, Irvine, CA 92697, USA. 3. Department of Mathematics, University of California, Riverside, CA 92521, USA; Interdisciplinary Center for Quantitative Modeling in Biology, University of California, Riverside, CA 92521, USA. 4. Department of Developmental and Cell Biology, University of California Irvine, Irvine, CA 92697, USA; Department of Mathematics, University of California Irvine, Irvine, CA 92697, USA; Department of Biomedical Engineering, University of California Irvine, Irvine, CA 92697, USA; Center for Complex Biological Systems, University of California Irvine, Irvine, CA 92697, USA; NSF-Simons Center for Multiscale Cell Fate Research, University of California Irvine, Irvine, CA 92697, USA. 5. Department of Developmental and Cell Biology, University of California Irvine, Irvine, CA 92697, USA; Department of Biomedical Engineering, University of California Irvine, Irvine, CA 92697, USA; Center for Complex Biological Systems, University of California Irvine, Irvine, CA 92697, USA; NSF-Simons Center for Multiscale Cell Fate Research, University of California Irvine, Irvine, CA 92697, USA. Electronic address: adlander@uci.edu.
Abstract
Gradients of decapentaplegic (Dpp) pattern Drosophila wing imaginal discs, establishing gene expression boundaries at specific locations. As discs grow, Dpp gradients expand, keeping relative boundary positions approximately stationary. Such scaling fails in mutants for Pentagone (pent), a gene repressed by Dpp that encodes a diffusible protein that expands Dpp gradients. Although these properties fit a recent mathematical model of automatic gradient scaling, that model requires an expander that spreads with minimal loss throughout a morphogen field. Here, we show that Pent's actions are confined to within just a few cell diameters of its site of synthesis and can be phenocopied by manipulating non-diffusible Pent targets strictly within the Pent expression domain. Using genetics and mathematical modeling, we develop an alternative model of scaling driven by feedback downregulation of Dpp receptors and co-receptors. Among the model's predictions is a size beyond which scaling fails-something we observe directly in wing discs.
Gradients of decapentapn>legic (n>an class="Gene">Dpp) pattern Drosophila wing imaginal discs, establishing gene expression boundaries at specific locations. As discs grow, Dpp gradients expand, keeping relative boundary positions approximately stationary. Such scaling fails in mutants for Pentagone (pent), a gene repressed by Dpp that encodes a diffusible protein that expands Dpp gradients. Although these properties fit a recent mathematical model of automatic gradient scaling, that model requires an expander that spreads with minimal loss throughout a morphogen field. Here, we show that Pent's actions are confined to within just a few cell diameters of its site of synthesis and can be phenocopied by manipulating non-diffusible Pent targets strictly within the Pent expression domain. Using genetics and mathematical modeling, we develop an alternative model of scaling driven by feedback downregulation of Dpp receptors and co-receptors. Among the model's predictions is a size beyond which scaling fails-something we observe directly in wing discs.
Authors: Kana Ishimatsu; Tom W Hiscock; Zach M Collins; Dini Wahyu Kartika Sari; Kenny Lischer; David L Richmond; Yasumasa Bessho; Takaaki Matsui; Sean G Megason Journal: Development Date: 2018-06-11 Impact factor: 6.868
Authors: Thomas Gregor; William Bialek; Rob R de Ruyter van Steveninck; David W Tank; Eric F Wieschaus Journal: Proc Natl Acad Sci U S A Date: 2005-12-13 Impact factor: 11.205