| Literature DB >> 32441935 |
Guanhua Xue1, Shaoli Li1, Weiwei Zhang1, Bing Du1, Jinghua Cui1, Chao Yan1, Lei Huang2, Lu Chen3, Linqing Zhao4, Yu Sun4, Nannan Li1, Hanqing Zhao1, Yanling Feng1, Zhimin Wang5, Shiyu Liu1, Qun Zhang1, Xianghui Xie6, Di Liu7, Hailan Yao8, Jing Yuan1.
Abstract
A novel coronavirus (SARS-CoV-2) was recently identified in patients with acute respiratory disease and spread quickly worldwide. A specific and rapid diagnostic method is important for early identification. The reverse-transcription recombinase-aided amplification (RT-RAA) assay is a rapid detection method for several pathogens. Assays were performed within 5-15 min as a one-step single tube reaction at 39 °C. In this study, we established two RT-RAA assays for the S and orf1ab gene of SARS-CoV-2 using clinical specimens for validation. The analytical sensitivity of the RT-RAA assay was 10 copies for the S and one copy for the orf1ab gene per reaction. Cross-reactions were not observed with any of the other respiratory pathogens. A 100% agreement between the RT-RAA and real-time PCR assays was accomplished after testing 120 respiratory specimens. These results demonstrate that the proposed RT-RAA assay will be beneficial as it is a faster, more sensitive, and more specific tool for the detection of SARS-CoV-2.Entities:
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Year: 2020 PMID： 32441935 DOI： 10.1021/acs.analchem.0c01032
Source DB: PubMed Journal: Anal Chem ISSN： 0003-2700 Impact factor: 6.986