| Literature DB >> 35132229 |
Liqian Wang1,2,3, Xuejun Wang1,2,3, Yungen Wu1,2,3, Mingquan Guo4, Chenjian Gu5, Changhao Dai1,2,3, Derong Kong1,2,3, Yao Wang5, Cong Zhang1,2,3, Di Qu5, Chunhai Fan6, Youhua Xie5, Zhaoqin Zhu7, Yunqi Liu3,8, Dacheng Wei9,10,11.
Abstract
The detection of samples at ultralow concentrations (one to ten copies in 100 μl) in biofluids is hampered by the orders-of-magnitude higher amounts of 'background' biomolecules. Here we report a molecular system, immobilized on a liquid-gated graphene field-effect transistor and consisting of an aptamer probe bound to a flexible single-stranded DNA cantilever linked to a self-assembled stiff tetrahedral double-stranded DNA structure, for the rapid and ultrasensitive electromechanical detection (down to one to two copies in 100 μl) of unamplified nucleic acids in biofluids, and also of ions, small molecules and proteins, as we show for Hg2+, adenosine 5'-triphosphate and thrombin. We implemented an electromechanical biosensor for the detection of SARS-CoV-2 into an integrated and portable prototype device, and show that it detected SARS-CoV-2 RNA in less than four minutes in all nasopharyngeal samples from 33 patients with COVID-19 (with cycle threshold values of 24.9-41.3) and in none of the 54 COVID-19-negative controls, without the need for RNA extraction or nucleic acid amplification.Entities:
Mesh：
Substances：
Year: 2022 PMID： 35132229 DOI： 10.1038/s41551-021-00833-7
Source DB: PubMed Journal: Nat Biomed Eng ISSN： 2157-846X Impact factor: 29.234