| Literature DB >> 32393822 |
Pranam Chatterjee1,2, Noah Jakimo3,4, Jooyoung Lee5, Nadia Amrani5, Tomás Rodríguez5, Sabrina R T Koseki3,4, Emma Tysinger3,4, Rui Qing4, Shilei Hao4, Erik J Sontheimer5, Joseph Jacobson3,4.
Abstract
CRISPR enzymes require a protospacer-adjacent motif (PAM) near the target cleavage site, constraining the sequences accessible for editing. In the present study, we combine protein motifs from several orthologs to engineer two variants of Streptococcus canis Cas9-Sc++ and a higher-fidelity mutant HiFi-Sc++-that have simultaneously broad 5'-NNG-3' PAM compatibility, robust DNA-cleavage activity and minimal off-target activity. Sc++ and HiFi-Sc++ extend the use of CRISPR editing for diverse applications.Entities:
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Year: 2020 PMID： 32393822 DOI： 10.1038/s41587-020-0517-0
Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908