Literature DB >> 34563399

Making the cut with PAMless CRISPR-Cas enzymes.

Kathleen A Christie1, Benjamin P Kleinstiver2.   

Abstract

Genome editing technologies simplify our ability to rewrite genetic blueprints of life. However, CRISPR-Cas enzymes found in nature can only manipulate a fraction of the genome. To overcome this limitation, new Cas variants have been developed that unlock nearly the entire genome for editing.
Copyright © 2021 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  CRISPR; PAM; genome editing; targeting range

Mesh：

Year:  2021        PMID： 34563399      PMCID： PMC9108897          DOI： 10.1016/j.tig.2021.09.002

Source DB:  PubMed          Journal:  Trends Genet        ISSN： 0168-9525            Impact factor:   11.821


  5 in total

1.  An engineered ScCas9 with broad PAM range and high specificity and activity.

Authors:  Pranam Chatterjee; Noah Jakimo; Jooyoung Lee; Nadia Amrani; Tomás Rodríguez; Sabrina R T Koseki; Emma Tysinger; Rui Qing; Shilei Hao; Erik J Sontheimer; Joseph Jacobson
Journal:  Nat Biotechnol       Date:  2020-05-11       Impact factor: 54.908

2.  A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.

Authors:  Martin Jinek; Krzysztof Chylinski; Ines Fonfara; Michael Hauer; Jennifer A Doudna; Emmanuelle Charpentier
Journal:  Science       Date:  2012-06-28       Impact factor: 47.728

3.  Engineered CRISPR-Cas9 nuclease with expanded targeting space.

Authors:  Hiroshi Nishimasu; Xi Shi; Soh Ishiguro; Linyi Gao; Seiichi Hirano; Sae Okazaki; Taichi Noda; Omar O Abudayyeh; Jonathan S Gootenberg; Hideto Mori; Seiya Oura; Benjamin Holmes; Mamoru Tanaka; Motoaki Seki; Hisato Hirano; Hiroyuki Aburatani; Ryuichiro Ishitani; Masahito Ikawa; Nozomu Yachie; Feng Zhang; Osamu Nureki
Journal:  Science       Date:  2018-08-30       Impact factor: 47.728

4.  Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.

Authors:  Russell T Walton; Kathleen A Christie; Madelynn N Whittaker; Benjamin P Kleinstiver
Journal:  Science       Date:  2020-03-26       Impact factor: 47.728

5.  Continuous evolution of SpCas9 variants compatible with non-G PAMs.

Authors:  Shannon M Miller; Tina Wang; Peyton B Randolph; Mandana Arbab; Max W Shen; Tony P Huang; Zaneta Matuszek; Gregory A Newby; Holly A Rees; David R Liu
Journal:  Nat Biotechnol       Date:  2020-02-10       Impact factor: 54.908
  5 in total

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