| Literature DB >> 32386599 |
Yufeng Lu1, Fion Shiau2, Wenyang Yi3, Suying Lu4, Qian Wu5, Joel D Pearson4, Alyssa Kallman6, Suijuan Zhong1, Thanh Hoang7, Zhentao Zuo8, Fangqi Zhao9, Mei Zhang10, Nicole Tsai11, Yan Zhuo8, Sheng He8, Jun Zhang9, Genevieve L Stein-O'Brien7, Thomas D Sherman12, Xin Duan11, Elana J Fertig13, Loyal A Goff14, Donald J Zack15, James T Handa6, Tian Xue16, Rod Bremner17, Seth Blackshaw18, Xiaoqun Wang19, Brian S Clark20.
Abstract
The development of single-cell RNA sequencing (scRNA-seq) has allowed high-resolution analysis of cell-type diversity and transcriptional networks controlling cell-fate specification. To identify the transcriptional networks governing human retinal development, we performed scRNA-seq analysis on 16 time points from developing retina as well as four early stages of retinal organoid differentiation. We identified evolutionarily conserved patterns of gene expression during retinal progenitor maturation and specification of all seven major retinal cell types. Furthermore, we identified gene-expression differences between developing macula and periphery and between distinct populations of horizontal cells. We also identified species-specific patterns of gene expression during human and mouse retinal development. Finally, we identified an unexpected role for ATOH7 expression in regulation of photoreceptor specification during late retinogenesis. These results provide a roadmap to future studies of human retinal development and may help guide the design of cell-based therapies for treating retinal dystrophies.Entities:
Keywords: cell fate; fovea; gene regulatory networks; neurogenesis; neurogenic bHLH factor; organoid; patterning; retina; single cell RNA-seq; transcription factors
Mesh：
Substances：
Year: 2020 PMID： 32386599 PMCID： PMC8015270 DOI： 10.1016/j.devcel.2020.04.009
Source DB: PubMed Journal: Dev Cell ISSN： 1534-5807 Impact factor: 12.270