Literature DB >> 3235672

Time-resolved fluorometry for the identification of viral DNA in clinical specimens.

P Dahlén1, P Hurskainen, T Lövgren, T Hyypiä.   

Abstract

Time-resolved fluorometry was used for the detection of DNA probes labeled directly with europium (Eu3+) chelate. The sensitivity of this nonisotopic hybridization assay was 100 pg of homologous DNA. Equivalent results with reference tests were obtained in the detection of adenoviruses in clinical specimens.

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Year:  1988        PMID： 3235672      PMCID： PMC266910          DOI： 10.1128/jcm.26.11.2434-2436.1988

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

1.  Novel chemical method for the preparation of nucleic acids for nonisotopic hybridization.

Authors:  R P Viscidi; C J Connelly; R H Yolken
Journal:  J Clin Microbiol       Date:  1986-02       Impact factor: 5.948

2.  Time-resolved fluorometry: a sensitive method to quantify DNA-hybrids.

Authors:  A C Syvänen; P Tchen; M Ranki; H Söderlund
Journal:  Nucleic Acids Res       Date:  1986-01-24       Impact factor: 16.971

3.  Non-radioactive nucleic acid probes for the diagnosis of virus infections.

Authors:  H G Pereira
Journal:  Bioessays       Date:  1986-03       Impact factor: 4.345

4.  Novel test for rapid viral diagnosis: detection of adenovirus in nasopharyngeal mucus aspirates by means of nucleic-acid sandwich hybridisation.

Authors:  M Virtanen; A Palva; M Laaksonen; P Halonen; H Söderlund; M Ranki
Journal:  Lancet       Date:  1983-02-19       Impact factor: 79.321

5.  Time-resolved fluorometer for lanthanide chelates--a new generation of nonisotopic immunoassays.

Authors:  E Soini; H Kojola
Journal:  Clin Chem       Date:  1983-01       Impact factor: 8.327

6.  Chemically modified nucleic acids as immunodetectable probes in hybridization experiments.

Authors:  P Tchen; R P Fuchs; E Sage; M Leng
Journal:  Proc Natl Acad Sci U S A       Date:  1984-06       Impact factor: 11.205

7.  A colorimetric method for DNA hybridization.

Authors:  M Renz; C Kurz
Journal:  Nucleic Acids Res       Date:  1984-04-25       Impact factor: 16.971

8.  Detection of adenovirus in nasopharyngeal specimens by radioactive and nonradioactive DNA probes.

Authors:  T Hyypiä
Journal:  J Clin Microbiol       Date:  1985-05       Impact factor: 5.948

9.  Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.

Authors:  J J Leary; D J Brigati; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205

10.  Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.

Authors:  P R Langer; A A Waldrop; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1981-11       Impact factor: 11.205
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  4 in total

1.  Time-resolved fluorometry PCR assay for rapid detection of herpes simplex virus in cerebrospinal fluid.

Authors:  V Hukkanen; T Rehn; R Kajander; M Sjöroos; M Waris
Journal:  J Clin Microbiol       Date:  2000-09       Impact factor: 5.948

2.  Detection of human immunodeficiency virus type 1 by using the polymerase chain reaction and a time-resolved fluorescence-based hybridization assay.

Authors:  P O Dahlén; A J Iitiä; G Skagius; A Frostell; M F Nunn; M Kwiatkowski
Journal:  J Clin Microbiol       Date:  1991-04       Impact factor: 5.948

3.  Detection of adenovirus in clinical specimens by polymerase chain reaction and liquid-phase hybridization quantitated by time-resolved fluorometry.

Authors:  J C Hierholzer; P E Halonen; P O Dahlen; P G Bingham; M M McDonough
Journal:  J Clin Microbiol       Date:  1993-07       Impact factor: 5.948

4.  Detection of human immunodeficiency virus type 1 RNA in plasma samples from high-risk pediatric patients by using the self-sustained sequence replication reaction.

Authors:  C E Bush; R M Donovan; W R Peterson; M B Jennings; V Bolton; D G Sherman; K M Vanden Brink; L A Beninsig; J H Godsey
Journal:  J Clin Microbiol       Date:  1992-02       Impact factor: 5.948
  4 in total

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