Literature DB >> 323226

Method for the isolation of Escherichia coli mutants with enhanced recombination between chromosomal duplications.

E B Konrad.   

Abstract

A method is described for the isolation of Escherichia coli mutants that show increased recombination between a pair of chromosomal duplications. These "hyper-rec" mutants display a variety of secondary phenotypes. I have isolated a large number of hyper-rec mutants and found them useful in screening for mutants that accumulate labeled DNA fragments after short pulses with [3H]thymidine. The mutants so recovered include ones that are defective in deoxyribonucleic acid ligase, deoxyribonucleic acid polymerase I and its associated 5' yields 3' exonuclease, and a group of mutants, dnaS, that accumulate abnormally short Okazaki fragments. Evidence is presented that suggests that the lac-att80 segment of the chromosome cannot be inverted.

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Year:  1977        PMID： 323226      PMCID： PMC235189          DOI： 10.1128/jb.130.1.167-172.1977

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  18 in total

1.  A DELETION ANALYSIS OF THE LAC OPERATOR REGION IN ESCHERICHIA COLI.

Authors:  J R BECKWITH
Journal:  J Mol Biol       Date:  1964-03       Impact factor: 5.469

2.  Genetic regulatory mechanisms in the synthesis of proteins.

Authors:  F JACOB; J MONOD
Journal:  J Mol Biol       Date:  1961-06       Impact factor: 5.469

Review 3.  Recalibrated linkage map of Escherichia coli K-12.

Authors:  B J Bachmann; K B Low; A L Taylor
Journal:  Bacteriol Rev       Date:  1976-03

4.  Genetic and enzymatic characterization of a conditional lethal mutant of Escherichia coli K12 with a temperature-sensitive DNA ligase.

Authors:  E B Konrad; P Modrich; I R Lehman
Journal:  J Mol Biol       Date:  1973-07-15       Impact factor: 5.469

5.  Ultraviolet-sensitive mutator strain of Escherichia coli K-12.

Authors:  E C Siegel
Journal:  J Bacteriol       Date:  1973-01       Impact factor: 3.490

6.  Genetics and function of DNA ligase in Escherichia coli.

Authors:  M M Gottesman; M L Hicks; M Gellert
Journal:  J Mol Biol       Date:  1973-07-15       Impact factor: 5.469

7.  Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division.

Authors:  Y Hirota; A Ryter; F Jacob
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1968

8.  Novel mutants of Escherichia coli that accumulate very small DNA replicative intermediates.

Authors:  E B Konrad; I R Lehman
Journal:  Proc Natl Acad Sci U S A       Date:  1975-06       Impact factor: 11.205

9.  Slow joining of newly replicated DNA chains in DNA polymerase I-deficient Escherichia coli mutants.

Authors:  R Okazaki; M Arisawa; A Sugino
Journal:  Proc Natl Acad Sci U S A       Date:  1971-12       Impact factor: 11.205

10.  Transient accumulation of Okazaki fragments as a result of uracil incorporation into nascent DNA.

Authors:  B K Tye; P O Nyman; I R Lehman; S Hochhauser; B Weiss
Journal:  Proc Natl Acad Sci U S A       Date:  1977-01       Impact factor: 11.205
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  86 in total

1.  Localized remodeling of the Escherichia coli chromosome: the patchwork of segments refractory and tolerant to inversion near the replication terminus.

Authors:  M I Guijo; J Patte; M del Mar Campos; J M Louarn; J E Rebollo
Journal:  Genetics       Date:  2001-04       Impact factor: 4.562

Review 2.  Historical overview: searching for replication help in all of the rec places.

Authors:  M M Cox
Journal:  Proc Natl Acad Sci U S A       Date:  2001-07-17       Impact factor: 11.205

3.  Genetic recombination during transformation in Bacillus subtilis: appearance of a deoxyribonucleic acid methylase.

Authors:  A T Ganesan
Journal:  J Bacteriol       Date:  1979-07       Impact factor: 3.490

4.  The phenotype of the minichromosome maintenance mutant mcm3 is characteristic of mutants defective in DNA replication.

Authors:  S I Gibson; R T Surosky; B K Tye
Journal:  Mol Cell Biol       Date:  1990-11       Impact factor: 4.272

5.  A partially deficient mutant, recA1730, that fails to form normal nucleoprotein filaments.

Authors:  M Dutreix; B Burnett; A Bailone; C M Radding; R Devoret
Journal:  Mol Gen Genet       Date:  1992-04

6.  Identification of a specific inhibitor for DNA ligase I in human cells.

Authors:  S W Yang; F F Becker; J Y Chan
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-15       Impact factor: 11.205

7.  Escherichia coli mutants deficient in deoxyuridine triphosphatase.

Authors:  S J Hochhauser; B Weiss
Journal:  J Bacteriol       Date:  1978-04       Impact factor: 3.490

8.  Context effects in the formation of deletions in Escherichia coli.

Authors:  T Kazic; D E Berg
Journal:  Genetics       Date:  1990-09       Impact factor: 4.562

9.  The uvp1 gene of plasmid pR cooperates with mucAB genes in the DNA repair process.

Authors:  F Gigliani; E Sporeno; S Perri; P A Battaglia
Journal:  Mol Gen Genet       Date:  1989-07

10.  UvrD303, a hyperhelicase mutant that antagonizes RecA-dependent SOS expression by a mechanism that depends on its C terminus.

Authors:  Richard C Centore; Michael C Leeson; Steven J Sandler
Journal:  J Bacteriol       Date:  2008-12-12       Impact factor: 3.490
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