| Literature DB >> 32273467 |
Qiang Huang1,2, Malkiel A Cohen3, Fernando C Alsina4, Garth Devlin5, Aliesha Garrett2, Jennifer McKey6, Patrick Havlik5, Nikolai Rakhilin2, Ergang Wang2, Kun Xiang2, Parker Mathews7, Lihua Wang2, Cheryl Bock8, Victor Ruthig6, Yi Wang2, Marcos Negrete2, Chi Wut Wong2, Preetish K L Murthy2, Shupei Zhang3, Andrea R Daniel9, David G Kirsch9,10, Yubin Kang7, Blanche Capel6, Aravind Asokan5, Debra L Silver4, Rudolf Jaenisch11,12, Xiling Shen13.
Abstract
Embryonic development is a complex process that is unamenable to direct observation. In this study, we implanted a window to the mouse uterus to visualize the developing embryo from embryonic day 9.5 to birth. This removable intravital window allowed manipulation and high-resolution imaging. In live mouse embryos, we observed transient neurotransmission and early vascularization of neural crest cell (NCC)-derived perivascular cells in the brain, autophagy in the retina, viral gene delivery, and chemical diffusion through the placenta. We combined the imaging window with in utero electroporation to label and track cell division and movement within embryos and observed that clusters of mouse NCC-derived cells expanded in interspecies chimeras, whereas adjacent human donor NCC-derived cells shrank. This technique can be combined with various tissue manipulation and microscopy methods to study the processes of development at unprecedented spatiotemporal resolution.Entities:
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Year: 2020 PMID： 32273467 PMCID： PMC7646360 DOI： 10.1126/science.aba0210
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728