Dynamic change of bacterial community during dairy manure composting process revealed by high-throughput sequencing and advanced bioinformatics tools.

The aim of this work was to study the dynamic change in structure and potential function of bacterial community during dairy manure composting process using high-throughput sequencing and advanced bioinformatics tools. Alpha diversity of microbial community significantly decreased during the thermophilic phase and then recovered gradually. Beta diversity analysis showed unique community structures in different composting phases. Keystone microbes such as genus Corynebacterium, Bacillus, Luteimonas and Nonomuraea were identified for different composting phases. Six functional modules were identified for bacterial community during the composting process using co-occurrence analysis. These modules were significantly associated with temperature, pH, degradation of organic matter and maturation of compost. Predicted metagenomics analysis showed that the relative abundance of amino acid, lipid, energy and xenobiotics metabolism increased during the composting process. These results provide valuable insights into the microbiota during dairy manure composting and how the structures and metabolic functions changed in response to composting phases.