| Literature DB >> 32139586 |
Ramona A Hoh1, Shilpa A Joshi1, Ji-Yeun Lee1, Brock A Martin1, Sushama Varma1, Shirley Kwok1, Sandra C A Nielsen1, Parastu Nejad1, Emily Haraguchi1, Priya S Dixit1, Swetha V Shutthanandan1, Krishna M Roskin2,3,4, Wenming Zhang5, Dana Tupa5, Bryan J Bunning5, Monali Manohar5, Robert Tibshirani6,7, Nielsen Q Fernandez-Becker8, Neeraja Kambham1, Robert B West1, Robert G Hamilton9, Mindy Tsai1,5, Stephen J Galli1,5,10, Rebecca S Chinthrajah5,11, Kari C Nadeau5,11, Scott D Boyd12,5.
Abstract
B cells in human food allergy have been studied predominantly in the blood. Little is known about IgE+ B cells or plasma cells in tissues exposed to dietary antigens. We characterized IgE+ clones in blood, stomach, duodenum, and esophagus of 19 peanut-allergic patients, using high-throughput DNA sequencing. IgE+ cells in allergic patients are enriched in stomach and duodenum, and have a plasma cell phenotype. Clonally related IgE+ and non-IgE-expressing cell frequencies in tissues suggest local isotype switching, including transitions between IgA and IgE isotypes. Highly similar antibody sequences specific for peanut allergen Ara h 2 are shared between patients, indicating that common immunoglobulin genetic rearrangements may contribute to pathogenesis. These data define the gastrointestinal tract as a reservoir of IgE+ B lineage cells in food allergy.Entities:
Mesh：
Substances：
Year: 2020 PMID： 32139586 PMCID： PMC7691169 DOI： 10.1126/sciimmunol.aay4209
Source DB: PubMed Journal: Sci Immunol ISSN： 2470-9468